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Of drug elimination liver Granule on acute liver injury in rats with LPS , TNF-alpha , IL -6 levels

Author: WangYaHong
Tutor: LiuTieJun
School: Changchun University of Traditional Chinese Medicine
Course: Chinese medical science
Keywords: Acute liver injury TCM dismount Experimental study Mechanism
CLC: R285.5
Type: Master's thesis
Year: 2009
Downloads: 100
Quote: 0
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Abstract


Objective: experimental observation of drug elimination liver clear particles of acute liver injury in rat liver function (ALT, AST), plasma toxin (LPS) and cytokines White mediated prime-6 (IL-6), tumor necrosis factor (TNF-alpha), the impact of changes in liver pathology, further clarify the drug elimination liver Granule on the effect and mechanism of prevention and treatment of acute liver injury. Methods: The rats were randomly divided into six groups: control group, model group, Mubarak control group for the alcohol, drug consumption liver Granule low, medium and high dose groups. Two pairs of groups (in addition to untreated and control group was given distilled water) the rest of the group were given of drug elimination liver Granule and lactitol bulk gavage for 7 consecutive days. Gavage 5 days in addition to the normal group, the rest of the group with the thioacetamide 600mg/kg intraperitoneal injection modeling method to establish the animal model of acute liver injury. All rats were killed after 48 hours of modeling, abdominal aortic blood collection, testing the animal serum enzymes in liver function and the use of enzyme-linked immunosorbent assay (enzyme linked immunosorbent assay, ELISA) to detect each group of rats with LPS, IL- 6, TNF-alpha content to observe the liver Granule on acute liver injury in rat liver function, LPS, IL-6, TNF-alpha level of drug consumption. 5 by electron microscopy poison consumer liver Granule acute liver injury in rat liver pathology. 6 drug consumption was observed liver Granule advance rate at the end of the small intestine of mice carbon. Results: 1 toxic liver elimination Granule acute liver injury in rat liver function, LPS impact: Compared with model group, the indicators of the serum of animals in each treatment group decreased significantly (P lt; 0.05); compared with the model group, drug consumption liver Granule, middle dose group animals liver function, LPS decreased significantly, there is a significant difference (P lt; 0.01), the preparation of acute liver injury in rat liver function, LPS and some improvement. 2 the drug elimination liver Granule on acute liver injury in rats IL-6, TNF-alpha content: Compared with model group, the drug elimination liver Granule high dose group animal serum TNF-a, IL-6 content decreased (P lt; 0.01), drug elimination liver Granule low-dose group animal serum TNF-a, IL-6 content decreased (P lt; 0.05), but no significant difference compared with the positive control group. The preparation of acute liver injury in rat TNF-a, IL-6 content of some improvement. Drug consumption liver Granule acute liver injury in rat liver pathological changes: the positive control group, high-dose, medium-dose and low-dose test drug group liver cell edema and necrosis is reduced significantly, indicating that the drug elimination hepatic clearance particles due to acute liver injury in animal models of liver injury in rats significantly reduced the protective effect. 4 toxic liver elimination Granule motility in mice: each group of animals administered carbon powder propelling percentage than the control group (P lt; 0.01), high the drug elimination liver Granule, the middle dose group and the positive control group was statistically significant compared to prompt the drug elimination liver Granule can significantly increase the animal motility. Conclusion: 1 600mg/kgTAA1 time intraperitoneal injection of modeling method can be successfully prepared animal model of acute liver injury. 2 Institute of determination of the indicators: toxic liver elimination Granule certain extent, protect liver function, reduce of LPS, IL-6, TNF-a level to improve the form of acute liver injury in rat liver pathology, high- efficacy of medium-dose group, and its overall efficacy is superior to the positive control group. 3 toxic liver elimination Granule bowel toxicity, diarrhea stool to reduce intestinal absorption of toxins, prevention and treatment of acute liver injury, and worthy of further research and development.

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