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Trehalose on Objective To evaluate the efficacy of evaporative dry eye rat model and to explore its therapeutic mechanism . Methods 55 SD rats ( 110 eyes) were randomly divided into three groups: normal group ( 5 of 10 eyes ) in the control group ( 25 of 50 eyes ) in the experimental group ( 25 total 50 eyes ) . Which normal group does not give any disposal , the control group and the experimental group to prevent rats blink mechanical , to exposure Keratoconjunctival 24 hours making evaporative dry eye model and modeling start were given sterile saline , 100 mmol / L trehalose solution droplets eyes , four times a day for 2 weeks . The model established immediately , 1 day , 3 Tian , 7 days , 14 Tian detection tear foundation secretion test ( Schirmer Ⅰ test , S Ⅰ t ) , tear film rupture time ( tear film the break - up time , BUT ) , fluorescein staining ( or fluorescence staining FLS), check to take dual corner of conjunctival tissue HE staining and immunohistochemical staining to histopathological examination keratoconjunctival and observation of the inflammatory cytokines TNF - α , IL - 6 expression . 1, S Ⅰ t comparison: the normal group, the control group and the experimental group three groups showed no significant difference ( P gt; 0.05 ) . 2 BUT : normal group group difference was no statistical significance ( P gt ; 0.05) ; experimental group and the control group, 7 days , 14 days difference was statistically significant ( P lt; 0.05 ) ; experimental group and the normal group, 14 days comparing the difference was statistically significant ( P lt; 0.01 ) . 3, FLS: Integral staining of the normal group (P gt ; 0.05) ; the control group and the experimental group in the model instantly Points have reached a high value , the control group, the observation period Diff statistical significance ( P gt ; 0.05 ) ; experimental group and the control group from day one integral group difference was statistically significant (P lt; 0.01); 4, immunohistochemistry revealed the results of comparison : the control group and the experimental group rat model to establish immediate corneal and conjunctival epithelial cells see a lot of expression of TNF-α, IL-6 , with the time of treatment , the control group, TNF - α , IL - 6 expression gradually reduce the experimental group, TNF - α , IL - 6 expression was significantly reduced . Normal group, the control group and the experimental group 14 days between the two groups , the differences were statistically significant ( P lt; 0.01 ) . Conclusion trehalose inhibits corneal and conjunctival epithelial inflammatory cytokines TNF - α , IL - 6 expression , reducing corneal and conjunctival surface inflammatory response , suggesting that trehalose application prospects of dry eye treatment .
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