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The Sensitivity Test of Gemcitabine to the Cell of Carcinoma of the Bladder

Author: YangPeng
Tutor: LiuZhenXiang
School: Central South University
Course: Surgery
Keywords: bladder cancer gemcitabine ATP-TCA individual chemotherapy
CLC: R737.14
Type: Master's thesis
Year: 2011
Downloads: 72
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Abstract


Objective:Gemcitabine is effective as single-agent or combined with Cisplatin in the treatment of advantage bladder cancer, it has become the first-line chemotherapy drugs for invasive bladder cancer.Literature has also permits gemcitabine could significantly inhibited many tumor cells in vitro> but the mechanisms for the induction of apoptosis by gemcitabine is not clear. This study was aimed at determining the molecular mechanisms and the efficacy of gemcitabine. ATP-TCA assay was used to identified the effect of GEM on the T24 bladder cancer cell lines and superficial bladder cancer cells in growth inhibition. Further definite the function mechanism of GEM from the cell level.Discussion whether the gemcitabine can significantly inhibited the bladder cancer cells in the growth,and whether for used to clinical chemotherapy of superficial bladder cancer. To investigate the heterogeneity of chemosensitivity in superficial bladder cancer using ATP-TCA assay. Observed the differences Sensitive rate between common chemotherapy drugs.Discussion the value of ATP-TCA assay on the patients with superficial bladder cancer.Methods:T24 bladder cancer cell lines were cultured in vitro. Use the ATP-TCA assay to detect the effect inhibition of growth of bladder cancer cells with different concentrations of gemcitabine. The morphological changes were observed through the optical microscope. From March 2010 to May 2011,20 superficial bladder cancer samples which received TUR-Bt were enrolled in the present study. Collected the Fresh bladder tumor specimens, separate the tumor cells and primary culture cells, to valuate the chemosensitivities of common anticancer drugs(Including gemcitabine) using ATP-TCA assay.Results:1. T24 bladder cancer cell lines resistance of different concentrations of gemcitabine.2. Gemcitabine affect T24 bladder cancer cell lines after a certain time, There is not significant apoptosis or necrosis.3.There is most resistant to gemcitabine used ATP-TCA assay to detect bladder cancer cells in different individuals.4.The sensitivity to chemotherapeutic drugs in different individuals were significantly,Single drug-sensitive rates were: THP(70.0%)>EPI(30.0%)>HCPT(20.0%)> GEM(20.0%)>MMC(10%)。5.The mean inhibiting rates of antitumor drugs in the primary group were notedly higher than that in the recurent group.Conclusions:1. There is no inhibition in the growth of Gemcitabine on the T24 bladder cancer cell line, and most of the resistant to bladder cancer cells in different individuals.2. ATP-TCA assay for drug sensitivity test in vitro might provide experimental basis for clinical individual chemotherapy of superficial bladder cancer.

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CLC: > Medicine, health > Oncology > Genitourinary tumors > Urinary tumors > Bladder tumor
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