|
Objective: 1 evaluation 20mg/kg genistein rabbit carotid intima exfoliative to autologous vein graft vascular remodeling (VR) intervention role. Explore the body vein graft 20mg/kg genistein vessel wall expression of MMP-2 and plasma NO, ET-1 levels in rabbit carotid endarterectomy self. Materials and methods: 1, animal model and packet 6-month-old adult male New Zealand white rabbits were 56 points rabbit carotid endarterectomy autologous vein graft ordinary diet group 28 rabbit carotid endarterectomy autologous vein graft 20mg/kg genistein intervention group 28. Rabbit left carotid endarterectomy anterior vein autogenous vein graft restenosis production model, contralateral sham-operated carotid artery and anterior vein as the control group, modeling After three days, seven days, 14 days and 42 days The four observation point in time, each 7-click model animal specimens from plasma after vascular specimens situ perfusion fixed drawn. 2, pathological data of image analysis morphometry analysis of specimens HE, Masson stained piece pathological image analysis system. Immunohistochemistry analysis after 3 days, 7 days, 14 days, 42 days immunohistochemical determination of the expression of MMP-2 in vascular specimens. 4, the chemical analysis respectively modeling and modeling after three days, 7 days, 14 days, 42 days lancing measurement of plasma levels of NO. Determination of nitrate by nitrate reductase projected level of plasma NO and the NO kit provided by Nanjing Jiancheng Bio, the testing of the blood samples from the same batch of reagents. 5, plasma ET-1 levels were measured by radioimmunoassay, using ET-1 kit Poor Albert Co., Ltd. from Beijing. Results: Genistein rabbit carotid artery intima stripping autologous vein graft vascular remodeling. Endarterectomy autologous vein graft was gradually narrowing trend in overall transplant venous lumen area (LA), 3-day group LA than in the control group was significantly increased, 7 days, 14 days, 42 days LA was significantly reduced, small seen in the control group and the formation of elastic fibers. 3 days genistein can inhibit the contraction of the vein graft remodeling, medication group LA than the control group, no significant changes in 14 days, 42 days LA than after ordinary diet group was significantly increased, effectively inhibit the contraction of vascular remodeling , there are statistically significant. 2 endarterectomy autologous vein graft anastomosis mouth proximal carotid artery, the external elastic lamina area overall was gradually reduced, after 3 days, 7 days anastomosis proximal carotid artery showed contraction remodeling, intimal The local formation; 14 days, 42 days coincide the mouth proximal carotid LA significantly reduced at the same time as the concurrent vascular contraction and the formation of plaque: genistein significantly inhibited the carotid artery contraction remodeling. Genistein intervention group than the control group on postoperative day 3 LA significant difference between day 7 internal and external elastic lamina retraction eased compared with the control group, 14 days, 42 days internal and external elastic lamina retraction significantly reduced compared with the control group, the smooth muscle cell proliferation to mitigate effectively inhibit contractile vascular remodeling. Genistein rabbit carotid artery intima stripping autologous vein graft MMP-2, NO, ET-1 expression in plasma NO levels after 3 days, 7 days, 14 days showed low expression, 42 the day than modeling before blood collection group, no significant difference. Genistein group 3 days, 7 days, 14 days was significantly higher than the normal diet group after surgery but lower than the model before blood collection group, statistically significant, and 42 days genistein group than made die before blood collection group no significant differences . Plasma ET-1 levels in autologous vein graft ordinary diet group showed high expression of 3 days was significantly higher after a decline in blood collection, 42 days prior to modeling group had no significant difference (P gt; 0.05). The genistein group 3 days, 7 days, after 14 days was lower than the normal diet group (P lt; 0.05), 42 days genistein group than before modeling blood collection group no significant difference (P gt; 0.05). 3 vascular wall expression of MMP-2 peak to 14 days. Vessel wall layers and MMP-2 expression rate has a significant correlation with the corresponding vascular remodeling. Genistein can inhibit the expression of MMP-2. Conclusion: In this study, successfully produced autogenous vein graft restenosis model rabbit carotid artery endarterectomy. 2, the process of restenosis artery remodeling phenomenon of shrinkage in the Presence of the stenosis of the lumen of the vascular remodeling depends index changes. The internal elastic lamina area (IELA) and external elastic lamina area (EELA) can be used as indicators of judgment stenosis and evaluation of vascular remodeling. Genistein can significantly reduce artery contraction remodeling. 3, the process of restenosis, vein graft exists the remodeling phenomenon of expansion and contraction, genistein significantly reduced venous contraction remodeling and intimal hyperplasia. 4, genistein can inhibit MMP-2, ET-1 generation, increased production of NO, reduce restenosis.
|