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Importance of MMP-9 and TIMP-1 on the Macrophage in Lack of Blood and Re-perfusion Model about Injured Rat Lung

Author: ZhiWeiWei
Tutor: GuoGuangWei
School: Shanxi Medical
Course: Department of Cardiothoracic Surgery
Keywords: Lung ischemia-reperfusion injury MMP-9 TIMP-1 Pulmonary macrophages
CLC: R655.3
Type: Master's thesis
Year: 2009
Downloads: 43
Quote: 0
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Abstract


Purpose according to the the Sekido prepared rat lung ischemia - reperfusion lung injury in animal models, from the molecular and genetic level observed macrophage-derived MMP-9 and TMIP-1 expression changes in rat lung ischemia - reperfusion injury early law, to explore its role in the pathogenesis of early ischemia - reperfusion lung injury therapy provides a new idea. Method 1. Subjects experimental animals using 48 healthy SD rats were randomly divided into six groups, each group of eight. Preparation of lung ischemia - reperfusion model 45min group, lung ischemia reperfusion group 30min reperfusion group 60min reperfusion group 120min reperfusion group 180min, respectively in ischemic 45min, the Reperfusion 30min, reperfusion 60min, and then perfusion the 120min, reperfusion 180min were sacrificed left lung tissue specimens, another blank control group. 2 with 37 ° C normal saline bronchial alveolar lavage, centrifugation, RPMI1640 medium containing 10% fetal calf serum to culture the cells were pelleted. By reverse transcription polymerase chain reaction (RT-PCR) was used to detect MMP-9 expression and TIMP-1mRNA the sandwich enzyme-linked immunosorbent assay macrophage-derived MMP-9 and TIMP-1 protein expression. And the obtained results were analyzed statistically. 1. Compared with the control group, ischemia 45min reperfusion for 30, 60, 120 min 180min group the MMP-9mRNA expression and protein concentrations were significantly increased, a significant difference (P lt; 0.05) reperfusion 120min mRNA expression and the protein concentration reached a peak. 2. Compared with the control group, ischemia 45min reperfusion for 30, 60, 120 min, 180min group the TIMP-1mRNA expression is significantly increased, there is a significant difference (P lt; 0.05), while the protein concentration decreased significantly, significantly difference (P lt; 0.05). 180min group the MMP-9mRNA expression and protein concentration reperfusion no significant increase compared with reperfusion 120min group; TIMP-1mRNA expression and protein concentration. Conclusion 1. Lung ischemia - reperfusion injury early elevated expression of MMP-9, and with the increase in ischemia - reperfusion time, MMP-9 lung ischemia-reperfusion injury during the formation of important inflammatory factor; MMP-9/TIMP-1 ratio increased, and with ischemia - reperfusion time, both the ratio imbalance is to produce ischemia - reperfusion-induced lung injury pathophysiological changes is an important reason, in certain extent, reflect the extent of both dynamic equilibrium of the body's condition and lung injury. 2. Compared with 120min reperfusion group, ischemia reperfusion 180min when no significant increase in the expression of MMP-9, TIMP-1 expression was significantly increased, suggesting that early lung injury in MMP-9 in lung ischemia - reperfusion should be early intervention, and to restore the MMP-9/TIMP-1 balanced to reduce inflammation.

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