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Study on Expression of Drug-resistance-associated Genes in Neoadjuvant Chemotherapy for Breast Cancer

Author: ZhouJianWei
Tutor: DengZuoYun
School: Nanchang University
Course: Oncology
Keywords: Breast Cancer Neoadjuvant chemotherapy P-gp GST-π Topo Ⅱ
CLC: R737.9
Type: Master's thesis
Year: 2009
Downloads: 139
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Abstract


Objective: to explore neoadjuvant chemotherapy in breast cancer tissue samples before and after resistance gene P-gp, GST-π, Topo II expression and its relationship with clinicopathological factors. Explore the relationship between P-gp, GST-π, Topo Ⅱ three resistance genes in breast cancer tissue specimens. 3, the analysis of the relationship between the three resistance genes in neoadjuvant chemotherapy in breast cancer tissue P-gp, GST-π, Topo Ⅱ. METHODS: P-gp monoclonal antibody, GST-π monoclonal antibody, Topo Ⅱ monoclonal antibodies using immunohistochemistry EnVison two-step, the Jiangxi Provincial Tumor Hospital from June 2006 to September 2008, menstrual Mammotome ( mammotome MT) biopsy confirmed 80 cases of primary breast cancer patients (both completed three cycles of neoadjuvant chemotherapy) detection puncture tissue cores before their chemotherapy and after surgery paraffin-embedded specimens. Results: 1, experiments into the group of 80 patients with breast cancer, completed after 3 cycles of chemotherapy neoadjuvant clinical partial remission (PR) 56 patients (70.0%), stable (SD) and 16 patients (20.0%), progress (PD) patients (10.0%), clinical effective rate of 70.0%, the total benefit rate of 90.0%. 2, GST-π in breast cancer tissue expression and patient age, tumor size, lymph node metastasis, clinical stage, ER, PR expression and Her-2 expression were not significantly different (P> 0.05); P-gp expression and ER PR expression was a significant negative correlation (P <0.05); Topo II expression and lymph node metastasis was significantly negatively correlated (P <0.01). Spearman rank correlation test between the three resistance genes before and after chemotherapy, expression of the strength of the correlation: P-gp and GST-π expression showed a significant positive correlation (P <0.01), Topo Ⅱ and P-gp, GST- π expression showed no correlation (P> 0.05). Χ2 test, before and after chemotherapy P-gp, of GST-π expression positive rate of change and expression intensity changes no noticeable significant difference (P> 0.05); while before and after chemotherapy Topo Ⅱ expression intensity changes have significantly with differences (P <0.01), before and after chemotherapy Topo Ⅱ positive expression rate of change was no significant difference (P> 0.05). 5, by χ2 test, chemotherapy before of P-gp, GST-π negative expression by chemotherapy efficiency significantly with higher than positive persons (P <0.05); Topo Ⅱ negative expression by chemotherapy have efficiency substantially with lower than the positive by (P <0.05). Conclusion: Neoadjuvant chemotherapy for stage Ⅱ, Ⅲ breast cancer can shrink the tumor size, prevention and treatment of micrometastasis to observe the sensitivity of tumor treatment, to provide guidance for postoperative adjuvant chemotherapy is the treatment of locally advanced breast cancer compared with good choice. 2, the experimental breast cancer new auxiliary chemotherapy before Topo Ⅱ expression and lymph node metastasis was very significant with negative correlation, of P-gp expression and ER, PR expression was significantly negatively correlated; chemotherapy before and after of P-gp, of GST-π and Topo Ⅱ positive expression rate of change was less affected, but a very significant change in the expression of Topo Ⅱ strength, increase the probability of resistance to chemotherapy; chemotherapy before and after the GST-π closely related to changes in the strength and intensity of the expression of P-gp, a similar adjustment mechanism may exist between . Breast cancer patients before chemotherapy combined detection of P-gp, GST-π, Topo II resistance gene expression, tumor chemotherapy drug of choice has a guiding role in predicting efficacy, determine prognosis and resistance reversal reference value.

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