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Experimental Study of the Chemotaxis Effect of Vegf in the Treatment of Cerebral Ischemia with Bone Marrow Stromal Cells

Author: HanJinLing
Tutor: YiLi
School: Shantou University
Course: Neurology
Keywords: Bone marrow stromal cells Cerebral ischemia Transplant Vascular endothelial growth factor
CLC: R743.3
Type: Master's thesis
Year: 2011
Downloads: 13
Quote: 0
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Abstract


Objective: Bone marrow stromal cells were isolated and cultured in vitro (BMSCs), 5 - bromodeoxyuridine (BrdU) in vitro labeled cells were observed at different time points mark rate ; intraluminal middle cerebral artery occlusion and reperfusion model (MCAO) , TTC staining and HE staining to determine cerebral ischemic area ; cisterna magna injection of BMSCs and vascular endothelial growth factor (VEGF), explore BMSCs MCAO cisterna magna injections of VEGF to its chemotactic effect. Methods : From 2 -month-old rats after limb bone marrow to take primary BMSCs, were cultured in vitro , identification , BrdU -labeled cells in vitro and observe the labeling efficiency . Suture method rat MCAO model (n = 30), 2h reperfusion . BMSCs after 24h treatment group (n = 10) and BMSCs VEGF treatment group (n = 10) cisterna magna injection 1 × 106个BMSCs, control group (n = 10) cisterna magna injection of saline . 48h, 72h, 96h component when BMSCs VEGF injections three times the cisterna magna VEGF20ng. Each group at 1 week and 4 -week time point from each of five rats were perfused brain was removed after fixed, paraffin -embedded , cut near the front fontanelle 2 4um thick brain slices were immunized with HE staining and BrdU histochemical staining different groups around the infarct BrdU immunohistochemical staining positive cells . Results : BrdU labeled BMSCs at 24h, 48h and 72h labeling efficiency was 43 %, 58 %, 96% . Saline group at each time point showed no positive cells . Two treatment groups at 1 week surrounding the infarct number of BrdU-positive cells when more than 4 weeks . In the treatment of one week and four weeks , the application of BMSCs VEGF treatment group than in the treatment group alone BMSCs infarct surrounding the number of BrdU-positive cells and more . P lt; 0.001, the difference was statistically significant . Conclusion : BrdU labeled BMSCs labeled 72h at a higher rate when the mark , reaching 96%. BMSCs cisterna magna injections rat MCAO , the transplanted cells migrated to the ischemic area , plus exogenous VEGF on BMSCs have to migrate around the infarct chemotactic effect.

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CLC: > Medicine, health > Neurology and psychiatry > Neurology > Cerebrovascular disease > Acute cerebrovascular disease ( stroke)
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