Dissertation > Excellent graduate degree dissertation topics show

The Establishment of the Novel Method to Detect Break-fusion Events Related with AML1 Gene

Author: LiQiuXing
Tutor: WangHongWei
School: Shanxi Medical
Course: Internal Medicine
Keywords: Acute leukemia Fusion gene AML1 AML1/ETO PCR
CLC: R733.7
Type: Master's thesis
Year: 2009
Downloads: 17
Quote: 0
Read: Download Dissertation

Abstract


0bjective To establish the novel method to detect break-fusion events related with AML1 gene and search new partner genes.Method (1)The AML1/ETO fusion gene was detected in 58 acute leukaemia patients by reverse transcriptase-polymerase chain reaction (RT-PCR). (2)The mRNA expression of AML1 gene break-point region was detected in 58 acute leukaemia patients and 26 controls by real quantitative reverse transcriptase-polymerase chain reaction (RQ-RT-PCR) and using a constantly expression region of AML1 gene as internal reference.Result (1)The frequence of acute leukemia with AML1/ETO was 23.5%(16/68). None of the 26 controls was found to carry AML1/ETO fusion gene.(2) The mRNA expression of the break-point region of AML1 gene in patients with AML1/ETO fusion gene was significantly lower than that in control group(P<0.05). (3)The mRNA expression of AML1 gene break-point region decreased significantly in 22 acute leukemia patients and the frequence was 32.3%(22/68). (4)In these 22 cases, the results of the two methods of 16 cases are coincident. We screened out 6 cases which maybe carry new partner genes. These cases include 2 AML-M2 cases, 4 AML-M4 cases.Conclusion The new method can detect efficiently more significant break-fusion events associated with AML1 gene. Combined with traditional RT-PCR method, the new method can be used as a efficient method which contribute to search its new partner genes.

Related Dissertations

  1. Cloning and Expression of CHS and CHI Genes and Their Regulation on the Accumulation of Flavonoids in ’Cara Cara’ Navel Orange (Citrus Sinensis Osbeck) and ’Guoqing NO.4’ Satsuma Mandarin (Citrus Unshiu Marcow),S666.4
  2. Studies on the Quality of Phisio-Chemical and Fresh Keeping Technologies of Post-Harvest in Volvariella Volvacea,S646.13
  3. Construction of the Recombinant of HSV-2gD Minotope、 HBsAg、 IL-18 DNA Vaccines and Its Inducing Immune Respons in Mice,R392
  4. Mutagenesis of a Laccase Gene from Pleurotus eryngii in Vitro and Expression in Pichia Pastoris,TQ925
  5. Gene Prediction and Sequence Analysis of Insect OBP CSP and sid-1,Q78
  6. Detection of Exogenous Recombinan DNA of Transgenic Rice and Its Rice Product,S511
  7. Isolationand Identification of Porcine Parvovirus and Parts of Its Biological Characteristics,S852.65
  8. Studies on Microbial Contamination and Microbial Decontamination of Chemical Substances of Fresh Chicken,TS251.1
  9. An Application of Real-Time PCR in the Study of Biological Control of Tobacco Wilt Disease,S435.72
  10. Ecological Control of Tobacco Bacterial Wilt in Continous-cropping Field and the Microecological Mechanisms,S435.72
  11. In Vitro Rapid Propagation System of Peach Yu Dwarfing Rootstock No.1 and Optimization of Its SSR-PCR System,S662.1
  12. Proliferation and Differentiation Effects of Different Culture Methods and Cell Factors on Mouse Spermatogenic Cells in Vitro,R329
  13. Effect of Probiotics on Growth Performance, Digestive Enzymes Activities, Immunity and Gut Microbiota of Epiniphelus Coioides,S917.4
  14. Study on the Physiological Differentiation and Genetic Diversity of Exserohilum Turcicum in Northeastern China,S435.131
  15. The DNA Cloning and RNA Interference of Some Functional Genes in Laodelphax Striatellus (Fallen),S435.112.3
  16. The Impact of Histone Deacetylase Inhibitor Trichostatin a on Proliferation and Apoptosis of Porcine Granulosa Cells,S828
  17. Effect of SO2 Exposure on DNA Methylation Polymorphism in Arabiadopsis Thaliana,Q943
  18. The Reform and Study Ofsite-directed Mutagenesis of Acid Xylanase XynⅢ from Aspergillus Niger,TQ925
  19. The Expression and Clinical Significance of TH17 Cells Related Cytokines in the Patients with Rheumatoid Arthritis,R593.22
  20. A Preliminary Study on Differentiation of Adipose-derived Stem Cell to Odontoblast-like Cell,R78
  21. Shanghai a porcine Cryptosporidium parasite identification and Zunyi City Cryptosporidium infection in humans and animals Preliminary Investigation,S855.9

CLC: > Medicine, health > Oncology > Hematopoietic and lymphoid neoplasms > Leukemia
© 2012 www.DissertationTopic.Net  Mobile