Dissertation > Excellent graduate degree dissertation topics show

PDTC in TRAIL Induced Raji Cells Apoptosis

Author: YanQingChun
Tutor: XiongXiaoLiang
School: Nanchang University
Course: Pathology and Pathophysiology
Keywords: PDTC TRAIL Raji cell Apoptosis
CLC: R733.1
Type: Master's thesis
Year: 2009
Downloads: 33
Quote: 0
Read: Download Dissertation

Abstract


Objective:To investigate whether PDTC could increase the Raji cells apoptosis induced by TRAIL and investigate the underlying mechanism.Methods:Using MTT assay to measure the cell growth inhibiting rate of Raji cell induced by TRAIL(1 ng/ml、10 ng/ml、100ng/ml )、PDTC(100μmol/ml)and TRAIL ( 1 ng/ml、10 ng/ml、100ng/ml ) combined with PDTC(100μmol/ml).The Raji cells apoptosis indix was examined by terminal deoxynucleotide transferas-mediated aUTP nick end labeling(TUNEL) .The expression of Membrane Proteins DR4、DR5 and nuclear protein NF-κB were detected by Western blot before and after the treatment of TRAIL、PDTC and co-treated with TRAIL and PDTC for 24h.Results:1. The 12h inhibiting rate of 1ng/ml、10ng/ml TRAIL was -35.52±5.64(%)and -15.07±2.03(%), the 12h inhibiting rate of 100ng/ml TRAIL was 6.68±1.17(%) and showed a time-dependent.The 12h inhibiting rate of 100μmol/mlPDTC was 1.01±0.21(%) and The 48 h inhibiting rate was 2.12±0.33(%),which showed a no significant inhibition on Raji cells.The 12 h inhibiting rate of TRAIL(1、10、100ng/ml)combined with the PDTC were 1.18±0.51、4.96±1.34、14.63±2.57(%),and were all markedly higher than in TRAIL and PDTC used alone(p<0.01).2. The co-treated group、TRAIL(100ng/ml)group and 100μmol/ml PDTC group after 48 h were all showed significantly different to control group(p<0.05).The highest apoptosis index of Raji cells induced by TRAIL combined with the PDTC was 79.49±1.40(%) which markedly higher than TRAIL100ng/ml(28.84±2.47%). The apoptosis index of Raji cell in 1ng/ml、10ng/ml TRAIL combined with the PDTC were also markedly higher than TRAIL and PDTC used alone (P<0.01)and also showed a time-dependent, which consistent of MTT.3.The expression of Membrane Proteins DR4、DR5 after treated with TRAIL(1、10、100ng/ml)for 24 h showed significantly diference in control group(P<0.05,p<0.01,p<0.01),but had no significantly difference in TRAIL co-treat with PDTC (p>0.05). The expression of nuclear protein NF-κB in control group is zero,but in TRAIL(1、10、100ng/ml) treated group was 0.7835±0.019、0.6607±0.0310、0.5194±0.024 which were all markedly higher than TRAIL co-treated with PDTC ( 0.3377±0.046、0.4837±0.012、0.6578±0.025). The Membrane Proteins DR4、DR5 and nuclear protein NF-κB at 100μmol/mlPDTC group were same as cotrol group.Conclusions:1. Raji cell is insensitive to TRAIL, PDTC could significantly increase the killing effect of TRAIL in Raji cell.2. The TUNEL show that PDTC primarily through increasing tumor cells apoptosis to enhance the killing effect of TRAIL.3. This study verified that TRAIL could establish a complex with death receptors and at the same time activate NF-κB.PDTC through significantly decrease the activation of NF-κB to increase the sensitiveity of TRAIL-induced apoptosis in Raji cell and the expression of death receptor not involved in this process.

Related Dissertations

  1. The Study on Cryopreservation and Mechanism of Freezing Injury on the Spermatozoa of Coelomactra Antiquate,S968.3
  2. The Anti-tumor Effect of CADPE and Inducing Apoptosis in Human Gastric Cancer Cells,R735.2
  3. Cloth and β- elemene combined administration of anti-tumor effect and mechanism of celecoxib,R96
  4. TRAIL in the regulation of tumor invasion CD4 ~ CD25 ~ Treg,R730.2
  5. The Study of Lead Exposure on Cyprinus Carpio Ovary Epithelial Cells,X174
  6. Effects of Diclazuril on G3PDH in Second-generation Merozoites of Eimeria Tenella,S858.31
  7. The Effects and Preliminary Study of PCV2 on Ca2+ Signal in Lymphocytes of Piglets,S858.28
  8. Effects of Angiogenic Factor on Follicular Angiogenesis and Development in Sexual Maturity Mice,S852.2
  9. Expression of β-Catenin in Pig’s Ovary and the Effect of β-Catenin on Porcine Granulosa Cells Apotosis and Steroidogenesis Related Enzyme,S828
  10. Induced Combination Apoptosis by FB1 and AFB1 in Vero Cell,S856.9
  11. The Apoptosis Mechanism Induced by Aflatoxin B1 and Deoxynivalenol in Primary Hepatocyte of Cyprinus Carpio,S856.9
  12. Effects of BMPR-IB Gene Silencing by Small Interfering RNA on Apoptosis of Porcine Pollicular Granulosa Cells and Ecpression of BMP Pathy-Way-Ralted Genes,S828
  13. The Effects of Don on Proliferation, Differentiation, and Apoptosis of Chondrocytes in Chicken,S858.31
  14. The Effect of Foxol on Apoptosis of Mouse Granule Cells,S865.13
  15. Study of Melittin on Tumor Inhibition and Part of Mechanism of Human Hepatocellular Carcinoma HepG-2 Xenograft in Nude Mice,R735.7
  16. The Protective Effect of Rat Myocardial Ischemia/reperfusion Injury Following Bone Marrow Mesenchymal Stem Cell Pretransplantation for 1 Week,R542.22
  17. Expression of WW Domain Containing Oxidoreductase Gene in Cholangiocarcinoma and Its Effect on the Biological Behavior of Cancer Cell Line QBC939,R735.8
  18. The Effects of (180F-FDG on the Apoptosis of Eca-109 Esophageal Cancer Cell Line,R735.1
  19. The Signal Mechanism Underlying DON Induced Apoptosis in BHK-21 Cells,S856.9
  20. The Expression and Mechanism of NF-κ B and IL-6 after 90% Portal Vein Ligation in Rats,R657.3
  21. The Study of Mitochondrial Signal Mechanism Induced Apoptosis by Don in Human Colon Cancer Cells,R735.35

CLC: > Medicine, health > Oncology > Hematopoietic and lymphoid neoplasms > Reticuloendothelial system tumors
© 2012 www.DissertationTopic.Net  Mobile