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Morphological Observation of Cholecystokinin-, Insulinand Glucagon-secreting Cells of Developing Pancreas in the Mouse

Author: LiYaQin
Tutor: WangTong
School: Shanxi Medical
Course: Human Anatomy and Embryology
Keywords: Mouse pancreas Development CCK - IR cells Ins - IR cells Glu - IR cells CCK - Ins - IR cells Ins - Glu - IR cells Double immunofluorescence method
CLC: R329
Type: Master's thesis
Year: 2009
Downloads: 49
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Abstract

Objective: To observe the developing the Kunming mouse pancreatic cholecystokinin immunoreactive cells (cholecystokinin immunoreactive cells, CCK-IR cells), insulin-immunoreactive cells (insulin immunoreactive cells, Ins-IR cells) and pancreatic glucagon immunoreactive cells ( Glucagon immunoreactive cells, Glu-IR cells) occurrence, distribution and morphological characteristics; cholecystokinin in the pancreas and insulin, insulin and glucagon coexist in the same cell phenomenon. Methods: Using immunohistochemistry SABC method was observed in mice embryos on day 11 after birth 45 days pancreas within CCK-IR cells, Ins-IR cells and Glu-IR cells occurred, distribution and morphological characteristics. And observed by immunofluorescence double staining cholecystokinin and insulin in the pancreas, insulin and glucagon hormone in the same cell, whether coexistence. Results: Immunohistochemical SABC method showed that mouse embryonic day 11, the pancreas is still in the early stages of differentiation, CCK-IR cells, Ins-IR cells and Glu-IR cells in the pancreas. Three kinds of cells often clustered distribution of light staining, cells ill-defined. To the embryo after 15 days, three cell coloring shades, cell boundaries are gradually discernible, were scattered in the islets. Late embryo to birth, deeply stained INS-IR cells and Glu-IR cell number increased Ins-IR cells in the islet there is a gradual diffusion-like distribution, Glu-IR cells in the islets scattered gradually towards the periphery. Only in the islets after birth even visible light staining of CCK-IR cells. Duct and acinar epithelial cells of the exocrine pancreas between embryonic period even see the three kinds of cells, but can be seen only after the birth of a mouse INS-IR cells and Glu-IR cells, and no CCK-IR cells. Immunofluorescence CCK-IR cells varied shapes, some cells can be seen extending elongated protrusion. CCK-IR cells in the embryo, the fluorescence intensity varies, after birth, the fluorescence gradually weakened, but can still be seen on the 45th day after birth, the edge portion of the islet CCK-IR cells. INS-IR cells and Glu-IR cells in the form of oval, round common. Embryonic day 15 before the weak fluorescence of the Ins-IR cells and Glu-IR cells, wherein the Glu-IR cells in the fluorescence intensity is stronger than Ins-IR cells. Embryo until late on the 45th day after birth, the fluorescence of two types of cells are gradually increased. Double immunofluorescent labeling were observed from the 45th day after the 14th day of the embryo to birth, always visible CCK-Ins-IR cells in the pancreas of mice, the number of late embryonic and postnatal day 30 more. The Ins-Glu-IR cells from embryonic day 11 to postnatal day 45 and mouse pancreatic visible, the number is up to 30 days embryo 11 days after birth. After birth occasionally visible Ins-Glu-IR cells in the exocrine pancreas. Conclusion: For display within the developing pancreatic CCK-IR cells by immunofluorescence than ordinary immunohistochemistry Chemical Technology is more sensitive. 2 CCK-IR cells in the developing mouse pancreas has more typical next to the secretion of cell morphology characteristics; prompt distribution period of the cells and CCK-IR cells may be the endocrine, next to secrete such as way affect islet endocrine cells and pancreatic exocrine secretion Ministry occurrence, development, and involved in the regulation of the secretory activity of the pancreas in mice after birth. 3 double immunofluorescence method can be used to show the coexistence of the two hormones in the same cell, this experiment CCK-Ins-IR cells, INS-Glu-IR cells may be naive endocrine pancreas cells. 4 islets within 30 days after birth, mice can be seen a large number of INS-Glu-IR cells, presumably, within the development and maturation of mouse pancreatic B cells may still have the potential to proliferate.

Morphological Observation of Cholecystokinin-, Insulinand Glucagon-secreting Cells of Developing Pancreas in the Mouse

Abstract

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