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Detection of the Expression Level of microRNA-140 (miR-140) in Knee Synovial Fluid of Osteoarthritis (OA) Patients Using Real-time Fluorescent Quantitative PCR

Author: ZhangMing
Tutor: XiaoTao
School: Central South University
Course: Surgery
Keywords: Osteoarthritis knee synovial fluid microRNA real-time quantitative PCR miR-140
CLC: R684.3
Type: Master's thesis
Year: 2011
Downloads: 195
Quote: 0
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Abstract


Objective:To study the potential function of miR-140 in develop-ment and progression of Osteoarthritis (OA) using real-time PCR.Materials and methods:The expression level of miR-140 in knee synovial fluid was detected by real-time PCR (stem-loop RT-qPCR, TaqMan). Knee synovial fluid specimens were divided into two groups: 1、the group of OA patients 40 cases(According to X-ray diagnosis criteria of Kellgren and Lawrence:gradeⅠ(10 cases)、gradeⅡ(10 cases)、gradeⅢ(10 cases)、gradeⅣ(10cases);2、the control group(20 cases)(meniscus injury 10 cases、cruciate ligament injury 5 cases、trauma 5 cases) patients. The expression levels of miR-140 in knee synovial fluid were detected by qPCR. The relationship between the severity of osteoarthritis and the expression levels of miR-140 was analysed. U6 snRNA was used as endogenous control.All data was analyzed by SPSS 17.0 statistical software.α=0.05 was regarded as the statistical criterion.Results:1. MicR-140 can be detected in all knee synovial fluid.2. The expression level of miR-140 in OA patients is much more lower compared with control group.(P<0.05).3. The expression levels of miR-140 and the severity of OA was negatively correlated.r=-0.917. Conclusion:1. There is miR-140 in human knee synovial fluid, which can be detected conveniently by real-time PCR.2. The expression level of miR-140 is lower in the knee synovial fluid of OA than control group,and have great correlation with the severity and progression of osteoarthritis(OA).

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CLC: > Medicine, health > Surgery > Orthopaedic Surgery ( movement system diseases,orthopedic surgery ) > Joint disease and injury > Arthritis
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