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Systemic lupus erythematosus CD4 ~ T cell IL-10 and IL-13 gene methylation status of regulatory sequences

Author: TangJinLing
Tutor: LuQianJin
School: Central South University
Course: Dermatology and Venereology
Keywords: Systemic Lupus Erythematosus CD4 ~ T cells IL-10 IL-13 Methylation
CLC: R593.241
Type: Master's thesis
Year: 2011
Downloads: 87
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Abstract


Objective : DNA methylation abnormalities in SLE ​​(Systemic lupus erythematosus, SLE) play an important role in the pathogenesis and interleukin (Interleukin, IL) IL-10 and IL-13 in patients with SLE autoantibodies and Th2 cell differentiation play an important role . However, the cause of SLE patients IL-10 and IL-13 overexpression of the mechanism is not clear. This study investigated patients with SLE CD4 T cells of IL-10 and IL-13 gene regulatory sequences and overexpression of the methylation status of the mechanism . Methods : Density gradient centrifugation normal , SLE patients with peripheral blood mononuclear cells , peripheral blood cells using immunomagnetic separation CD4 T cells , adding 1μM methylation inhibitor 5 - aza Cellular Effects (5-azaC), in RPMI 1640/10% FCS/IL-2 incubated for at least 72h. Real-time quantitative RT-PCR (real-time RT-PCR) detection of CD4 T cells, IL-10 and IL-13 mRNA levels ; enzyme-linked immunosorbent assay (ELISAs) serum IL-10 and IL-13 protein level ; bisulfite sequencing assay IL-10 and IL-13 gene regulatory region methylation status . Results: Compared with normal control group , SLE patients with CD4 T cell IL-10 and IL-13mRNA levels were significantly higher (IL-10: P = 0.001; IL-13: P = 0.012); ELISA in serum of SLE patients showed IL-10 and IL-13 protein levels were significantly increased (IL-10: P = 0.005; IL-13: P = 0.007); bisulfite sequencing results showed that SLE CD4 T cell IL-10 and IL-13 gene regulation region DNA methylation levels were significantly lower (IL-10: P = 0.036; IL-13: P = 0.006), and respectively with IL-10 and IL-13 mRNA expression was significantly negatively correlated (IL-10: p = 0.04; IL-13: P = 0.006); DNA methylation inhibitor 5-azacytidine (5-azaC) handle the normal CD4 T cells increased IL-10 and IL-13 mRNA expression (IL-10: p = 0.017; IL-13: p = 0.025). CONCLUSION : DNA methylation involved in regulation of CD4 T cells in SLE patients of IL-10 and IL-13 expression ; SLE patients with IL-10 and IL-13 regulatory sequence hypomethylation in the development of SLE play an important role .

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CLC: > Medicine, health > Internal Medicine > Systemic disease > Autoimmune diseases > Autoimmune diseases, connective tissue disease > Lupus erythematosus > Systemic lupus erythematosus
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