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Objective: In animal models of type 2 diabetes used during the preparation of 1,25 - (OH) 2 vitamin D3 [1,25 - (OH) 2D3] intervention study its effect on type 2 diabetic rats adipokines and inflammatory factors and to explore 1,25 - (OH) 2D3 in rats with type 2 diabetes in rats. METHODS: Eight -week-old male SD rats were randomly divided into normal control group, diabetic 1,25 - (OH) 2D3 intervention group ( intervention group diabetes VD ) , diabetes non 1,25 - (OH) 2D3 intervention group ( non- diabetic VD intervention group ) . After 6 weeks of diabetes VD VD intervention and non- intervention group were given diabetic streptozotocin (streptozotocin, STZ) 30mg/kg intraperitoneal injection to establish animal models of type 2 diabetes . The incidence of diabetes observed after one week , each group was measured body weight , fasting glucose, triglycerides, total cholesterol, calcium, phosphorus, serum by radioimmunoassay fasting insulin (insulin), leptin (leptin), tumor necrosis factor -α (tumor nectrosis factor-a, TNF-a), interleukin -6 (interleukin-6, IL-6), ELISA method (enzyme linked immunosorbent assay) serum adiponectin (adiponectin, ADP), RT-PCR method (reverse transcription polymerase chain reaction) detection of adipose tissue adiponectin mRNA. Results: 1 ) compared with normal control group , diabetic rats ( including non- intervention group and VD VD intervention group ) fasting glucose , fasting insulin , total cholesterol, leptin , serum calcium , phosphorus were significantly increased , diabetes, non- VD intervention group were triglycerides , IL-6, TNF-α levels, serum adiponectin and adipose tissue adiponectin mRNA levels were significantly lower ( both P lt; 0.05); 2) the intervention group and non- diabetic VD VD intervention group, fasting insulin , IL-6, TNF- significantly decreased serum adiponectin and adipose tissue adiponectin mRNA levels (P lt; 0.05); 3) fasting blood glucose , triglycerides, total cholesterol , thin hormone, calcium, phosphorus intervention in diabetic and diabetic non- VD VD intervention group showed no significant difference between (P gt; 0.05); 4) diabetes VD VD intervention and non- intervention group, diabetes incidence equal. Conclusion : 1,25 - (OH) 2D3 by reducing TNF-α, IL-6, increased adiponectin (?) NRNA level, from the molecular level on the incidence of type 2 diabetic rats play a protective role .
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