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Bcl-xL and Fas Protein Change in Spermatogenic Cells of OLETF Rat Testes and Rosiglitazone Intervention Effect

Author: HuaChao
Tutor: LiuZuo
School: Tianjin Medical University
Course: Human Anatomy and Embryology
Keywords: Type 2 diabetes mellitus Testis Rosiglitazone OLETF rat Bcl-xL Fas protein
CLC: R587.1
Type: Master's thesis
Year: 2011
Downloads: 25
Quote: 0
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Abstract


The purpose of diabetes (diabetes mellitus, DM) is a chronic, lifelong disease, with the average life expectancy of the people, the improvement of living standards and changes in the structure of diet, the prevalence of diabetes is increasing. Diabetes has become a chronic non-communicable diseases following the third-largest after cancer, cardiovascular serious threat to human health. Reproductive system dysfunction is one of the complications of diabetes, the incidence of non-diabetic patients 5-10 times, serious pressure and distress to the patient's physical and mental health, more and more attention in recent years. Diabetes reproductive system dysfunction, including erectile dysfunction (erectile dysfunction, ED) and male infertility, etc.. OLETF rat is a spontaneous type 2 diabetic rats, the experimental as type 2 diabetes model OLETF rats, LETO rats and photo Ruluo Ge rosiglitazone OLETF rats as control, micro-pathological changes observed testicular and spermatogenic apoptosis and epididymal sperm by the energy, and the use of immunohistochemical methods to detect the spermatogenic cell apoptosis-related protein expression of Bcl-xL and Fas, and to explore the mechanism of the occurrence of diabetes reproductive system dysfunction and rosiglitazone intervention. Rats under specific pathogen-free (SPF grade) single cages, fed a standard diet, 12/12h light-dark cycle, free access to food and drinking water. Regularly the line oral glucose tolerance test (OGTT) monitoring of blood glucose, blood glucose peak gt; 16.7mmol / L, and 120 min after load blood glucose gt; 11.1mmol / L, a diagnosis of diabetes, only have the one with impaired glucose tolerance. Into a mold OLETF rats 12 to 30 weeks, a total of diabetic control (DM) were randomly divided into groups and rosiglitazone (RGZ) group (n = 6), 8 LETO rats as normal control (NC) group . Rosiglitazone dissolved in distilled water diluted RGZ rats administered orally at a dose of 3mg/kg · d. DM group and NC group distilled water to the same amount in each group are fed daily to 12 weeks. Weighing the body weight of rats after 12 weeks, then dislocated the rats were killed, lower abdominal midline incision in the abdominal wall, remove the rat testis, rinse with cold saline, filter paper for drying, accurately weighed. Then placed in 10% neutral formalin solution fixed, paraffin-embedded sections, HE staining. Light microscope to observe the change of the organizational structure, measuring about morphological indicators. Acridine acridine orange staining evaluation attached Chui sperm fertilization capacity. The end of the ISEL in situ nick translation method for detecting spermatogenic cells apoptosis. Determination of Bcl-xL application SABC (strept avidin-biotin complex) method, brown granules appear in the cytoplasm or (and) membrane as a positive expression of anti PBS instead as a negative control. Fas protein expression changes detected in accordance with the SP method. The images were analyzed using image analysis software Motic Images Advanced3.2 version. Results 1. Modeling: OLETF rats compared with LETO the obviously obese rats, polydipsia, polyphagia, polyuria, diabetes clinical performance. 2. Testicular gross morphology and weighing: visual observation, model group testicular obvious atrophy, severe testicular collapse soft, dark red in color, texture unclear minority testicular albuginea rupture. Rosiglitazone group testicle testicle with the model group visual observation of little change. Testicular weight changes, compared with the model group, the control group, testicular weight larger than the model group the rosiglitazone group testicular weight and model group difference was not statistically significant. Testicular tissue HE staining were observed: the model testis than in the control group had a significant pathological changes. Rosiglitazone group, seminiferous tubule spacing widened the spermatogenic cell disorder, the rare sperm within the lumen. Compared with the control group, the model group spermatogonia and interstitial cell count decreased; rosiglitazone group compared with the model group, there is no statistically significant difference. Testicular germ cell apoptosis changes: compared with the diabetic group, spermatogenic cell count is less than the control group, testicular apoptosis diabetic group, the rosiglitazone group and diabetes group had no significant difference. 5 spermatogenic cell expression of Bcl-xL protein: Compared with model group, the control group spermatogenic cell protein expression of Bcl-xL average gray values ??decrease, rosiglitazone ketone group spermatogenic cells Bcl-xL protein expression average gray value model was no statistically significant difference. 6. Spermatogenic cell Fas expression changes: mainly to weak expression of Fas protein in the control group, and strong positive expression in the model group, mainly moderately positive expression in the rosiglitazone group. Conclusion 1. OLETF rats showed diabetes similar, can be used as an ideal animal model of type 2 diabetes. 2. OLETF rat testis significantly shrink or even collapse. Testicular coefficient decreases. Light microscope, pathological changes were performance living a decrease in the number of seminiferous tubules, atrophy distorted basement membrane damage, reduce the number of spermatogenic cells, connective tissue proliferation, mesenchymal stromal cells decreased. 3. OLETF rat testicular germ cell apoptosis increased, decreased epididymal sperm fertilizing capacity. Apoptotic mechanisms may be involved in type 2 diabetes spermatogenic failure. Rosiglitazone can reduce the blood sugar of type 2 diabetes, but the cause of type 2 diabetes spermatogenic failure did not improve the effect.

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CLC: > Medicine, health > Internal Medicine > Endocrine diseases and metabolic diseases > Islet disease > Diabetes
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