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The Screening and Evaluation of Generated Biopolymer Bacterium

Author: WangChaoXia
Tutor: WuJingChun
School: Daqing Petroleum Institute
Course: Oil and Gas Field Development Project
Keywords: Biopolymer Screening of Strains Medium Profile control Oil recovery
CLC: TE357.4
Type: Master's thesis
Year: 2008
Downloads: 80
Quote: 1
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Abstract


Biopolymer has many excellent performance in recent years , often used as a displacing agent profile control agent and drilling fluids . However, the the strain screening study of biopolymers under reservoir conditions is still relatively small. Looking for more environmental adaptability , yield higher strains , the development of more temperature, more stable biopolymer is the most fundamental solution to the problem of microbial scholars at home and abroad committed by one of the research project . In this paper , the core issue of biological polymer , strain selection , fermentation , and the performance evaluation of the combination of research methods . Strains isolated from the Daqing oil field oil-water mixed samples screened one has a produce biopolymers performance , short fermentation period (numbered the BH 8 < / sub > ) . The strain was facultative anaerobes and reservoir good compatibility with the Daqing Oilfield . Experiments to determine the optimum conditions for fermentation bacteria : incubation temperature to 45 ° C , ventilation of 0.2m 3 < / sup > / h , the pH value of 7 to 8 . Select the type of carbon source, a nitrogen source , mineral , nutrient elements in the preparation of the medium , the the BH 8 < / sub > was inoculated to each medium for fermentation culture , the viscosity of the fermentation broth were measured , and selection allows the viscosity of the fermentation broth medium composition reaches a maximum . Orthogonal experimental method to analyze the impact of the medium formulation factors , different levels of viscosity of fermentation broth , to obtain the maximum level of each factor to determine the optimum medium for bacteria fermentation component . The BH8 bacteria in porous media grew well metabolite of biopolymers . BH 8 < / sub > bacteria plugging high permeability layer , profile control experimental results show that the biopolymer injection core effectively block the high permeability layer , making the injection pressure profile control has improved significantly . Interior coreflood The results showed that in after waterflooding to 98% aqueous , will the BH 8 is Place bacteria and nutrient solution injection cores 3d, subsequent water flooding , can improve the recovery factor of 10 percentage points.

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CLC: > Industrial Technology > Oil and gas industry > Oil and gas development and mining > Production Engineering > Enhanced oil recovery and maintain reservoir pressure (secondary and tertiary oil recovery ) > Thermal, miscible , Chemical Oil Recovery (EOR EOR )
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