Dissertation > Excellent graduate degree dissertation topics show

The Study of Both Magnetic Resonance Diffusion-weighted Imaging and Expression of VEGF Compared with Pathology in Dog Model of Liver Fibrosis

Author: LiCongRui
Tutor: WangWei
School: Central South University
Course: Medical Imaging and Nuclear Medicine
Keywords: Liver fibrosis magnetic resonance imaging diffusion-weighted imaging vascular endothelial growth factor animal model
CLC: R575.2
Type: Master's thesis
Year: 2011
Downloads: 49
Quote: 0
Read: Download Dissertation

Abstract


OBJECTIVE In our study, magnetic resonance diffusion-weighted imaging (DWI) and vascular endothelial growth factor (VEGF) immunohistochemical staining were underwent in dog model of liver fibrosis.The changes of them in different stage of liver fibrosis were analyzed.DWI and VEGF immunohistochemical staining results were compared with histopathological features to make further interpretations.As well as the correlations among them were analyzed,so as to choose method for assessing liver fibrosis reasonablely,to improve the diagnosis of hepatic fibrosis.METHODS 12 healthy male dogs were randomly divided into 2 groups, 10 dogs in experimental group,2 dogs in control group.0.125ml/kg saline were injected in peritoneal cavity twice a week in dogs of control group, while in experimental groups were injected 0.125ml/kg 50% of the carbon tetrachloride (CCL4) oil solution twice a week, and were received the high fat diet. The experimental group and control group were underwent MR examination and liver biopsy every 4 weeks respectively. All MR examinations were performed on a Siemens 1.5T unit, scanning sequences included:conventional T1WI, T2WI scan, then underwent DWI.Image postprocessing was performed on magnetic resonance image matching software, to obtain ADC value and EADC value.After each MR scan, biopsying of liver in dogs under the guiding of ultrasound, the liver tissue were underwent pathology routine HE staining, Masson staining and immunohistochemistry VEGF staining. The liver tissue samples were divided into 5 groups by the results of pathological stage, namely, S0-S4, the level of VEGF expression, the corresponding ADC values and EADC values were compared among different stages of liver fibrosis, analyzing the correlations among them.RESULT The experimental successfully received models of liver fibrosis:S0=12 cases, S1=9cases, S2=9cases, S3=10cases, S4=10cases. There Was a decreasing(increasing) trend in hepatic ADC(EADC) values with increasing degree of fibrosis. One-way ANOVA test was used to compare the difference of ADC(EADC) value among fibrosis stage. It showed there were significant differences among different fibrosis stages at ADC(EADC) value (P<0.01). The Spearman rank correlation test was used to assess the correlation both liver ADC values and EADC values and stage of fibrosis. There was negative(positive) correlation between stage of fibrosis and ADC(EADC) values of DWI images(P<0.05). VEGF expression increased with increaseing degrees of liver fibrosis.CONCLUSIONS An intraperitoneal injection of carbon tetrachloride in oil solution with the high fat diet in dogs can be established different stages of liver fibrosis. DWI imaging is a non-invasive technology and could be operated easily, and ADC value measurement has better reproducibility.DWI could provide a reference in early diagnosis,treatment and follow-ups for chronic liver disease. The value of ADC(EADC) can quantitatly analysis deferent degree hepatic fibrosis,the size of ADC(EADC) had negative(positive) correlation with degree of hepatic fibrosis.VEGF may play an important role in the process of liver fibrosis in chronic liver disease.

Related Dissertations

  1. Research of the Correlation About the Expression of Mfap4 in Liver Fibrosis Tissues and Concentrations of Mfap4 in Peripheral Blood Associated with Liver Pathological Grade,R575.2
  2. The Clinic Value of Diffusion Weighted Whole Body Imaging with Background Body Signal Suppression (DWIBS) Compared with hPET/CT,R445.2
  3. Treatment of Device-Related Infection in Rabitts by High Requency Electric,R687.3
  4. The Expression of MMP-2、 VEGF and Ki-67 and Human Glioma Grade,R739.41
  5. The Value of Diagnosis for Prostate Cancer by Combined Use of MRS and DWI,R737.25
  6. Expression Change of Signaling Molecule Related Wnt Signal Pathway in Mouse Cleft Palate Induced by Retinoic Acid During Perinatal Stage,R782.21
  7. The Research on Treatment of Lentivirus-mediated hVEGF-165 Gene Transferring Mesenchymal Stem Cells on Rat Hind Limb Ischemia,R329
  8. Study on Expression of MMP-8 on Experimental Periapial Periodontitis is Infected by Enterococcus Faecalis,R781.34
  9. Experiment on Repeated Bone Harvest Chamber in Vivo,R329
  10. BMP2 and VEGF165 Genes Transfected Rat Bone Marrow Mesechymal Stem Cells Simultaneity Expression,R329
  11. Study of MRI Diffusion-weighted Imaging in the Diagnosis of Lymphadenopathy and Evaluation after Radiotherapy,R445.2
  12. Serum Cholinesterase, Platelet, Liver Fibrosis HBV Infection in Various Stages of Clinical Significance,R575.2
  13. Piglet Hypoxic Ischemic Encephalopathy of DWI Images,R722.1
  14. Healthy Chinese central olfactory structures related to MRI volume and olfactory function with age correlation,R445.2
  15. Diffusion -weighted imaging and dynamic contrast-enhanced scan in cervical cancer staging and therapeutic monitoring applications,R737.33
  16. 3.0T high-field magnetic resonance imaging of the central type lung cancer preliminary study,R734.2
  17. MR diffusion weighted imaging in Application Value of osteonecrosis,R681.8
  18. A Preliminary Exploration of Laboratory Diagnosis and IVF Improvement Protocol on Endometriosis,R711.71
  19. Application Study on Quantitative Analysis of Diffusion-weighted Imaging and Dynamic Contrast-enhanced MRI in Thediagnosis of Breast Lesions,R445.2
  20. Application of Functional MR Imaging in Diagnose for Prostate Cancer,R737.25
  21. Expression and Significance of Breast Cancer Resistance Protein and Topoisomerase Ⅱ in Nude Mice Transplanted Tumor Model of A549/CDDP Multidrug Resistance Cell,R730.5

CLC: > Medicine, health > Internal Medicine > Digestive and abdominal diseases > Liver and gall bladder disease > Cirrhosis
© 2012 www.DissertationTopic.Net  Mobile