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Expressions of Rac1 in Normal and Aminoglycoside-induced Ototoxic Damaged Cochlea of Guinea Pigs

Author: LiaoJun
Tutor: WuWeiJing
School: Central South University
Course: Otorhinolaryngology
Keywords: Rac1 Cochlea Gentamicin Ototoxicity Antioxidants Sodium salicylate
CLC: R764
Type: Master's thesis
Year: 2009
Downloads: 21
Quote: 0
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Abstract


Objective To study the gentamicin ear toxic injury and application of anti- oxidant sodium salicylate protection Rac1 expression in the guinea pig cochlea , to investigate the role of Rac1 aminoglycosides ototoxicity mechanism . Methods Male albino guinea pigs to 30 , were randomly divided into three groups . Ⅰ group ( control group ) : intraperitoneal injection of saline for 7 consecutive days ; Ⅱ group ( gentamicin group ) : intraperitoneal injection of gentamicin sulfate , for seven consecutive days ; Ⅲ group ( gentamicin salicylic acid sodium group ) : intraperitoneal injection of gentamicin sulfate and sodium salicylate , for seven consecutive days; paraffin section immunohistochemistry to detect each group guinea pig cochlea Rac1 protein expression and distribution ; extract cochlear protein by Western blot to detect each group Rac1 protein expression levels . (1) immunohistochemical staining : control group cochlear spiral and spiral ganglion cells Rac1 weakly positive expression , mainly expressed in the cytoplasm and cell membrane ; the gentamicin group cochlea Rac1 increased expression ; gentamicin salicylic acid sodium group positive expression , the expression level between the control group and between the gentamicin group . Each group gradation value has a significant difference ( P <0.05 ) . (2) Western blot test: control group cochlea Rac1 protein expression of low intensity ; strongest gentamicin group cochlea Rac1 protein expression ; gentamicin salicylic acid the sodium group cochlea Rac1 protein expression levels between the control group and the big celebration ADM group between . Among the groups integrated optical density value of protein electrophoresis significant difference ( P <0.05 ) . Allows the Rac1 expression than celebrate conclusion Rac1 weak expression in normal guinea pig cochlea and spiral ganglion cell cytoplasm and membrane in the cochlea after gentamicin administration Rac1 expression increased , while giving antioxidants sodium salicylate the large ADR group weakened. These results suggest that Rac1 may play a role in cochlear injury led to gentamicin .

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CLC: > Medicine, health > Otorhinolaryngology > Otology,ear disease
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