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RhoGDIa on lung cancer cell apoptosis and related signaling proteins in

Author: RongFeng
Tutor: TaoMin
School: Suzhou University
Course: Oncology
Keywords: Lung cancer RhoGDIα Apoptosis Chemosensitivity RNA interference Western blot Immunohistochemistry
CLC: R734.2
Type: Master's thesis
Year: 2009
Downloads: 35
Quote: 0
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Abstract


Objective: To investigate RhoGDIα apoptosis of lung cancer and chemotherapy sensitivity and signaling pathways may be involved in order to understand the possible mechanism of apoptosis in lung cancer cells for the treatment of lung cancer to find new targets. Method: 1. Application RhoGDIαsiRNA \P-ERK, P-JNK, P-BAD and P-IκBα protein expression levels change; 2. detection RhoGDIα knockdown A549 cells after induction of apoptosis in response to paclitaxel and related signaling proteins change; 3 In order to further In vitro cell studies confirmed the conclusions obtained on 40 cases of lung tissue were detected by immunohistochemical changes in protein expression levels above, and RhoGDIα proteins and associated signaling protein expression correlation analysis. Results: 1. \lt; 0.05) and P-BAD, P-IκBα and P-ERK expression level decreased respectively (P lt; 0.05); 2. paclitaxel-induced A549 cells G2 / M phase arrest and apoptosis, low concentrations of paclitaxel (10nM ) induced apoptosis of A549 cells increased 2-fold (3.22% ± 0.16% vs 1.52% ± 0.14%, P lt; 0.05), accompanied by P-ERK and P-IκBα expression levels respectively (P lt; 0.05); 3 . RhoGDIαsiRNA transfected A549 cells were treated with low concentrations of paclitaxel (10nM) treatment, increased apoptosis 8 times (12.49% ± 1.03% vs 1.52% ± 0.14%, P lt; 0.05), accompanied by the expression level of P-JNK l high (P lt; 0.05) and P-BAD, P-IκBα and P-ERK expression levels decreased (P both lt; 0.05); 4. in lung cancer, RhoGDIα protein expression levels were significantly higher than in adjacent tissues ( 85% vs 60%, P = 0.0267), RhoGDIα protein expression levels and P-BAD expression levels positively correlated (r = 0.459, P = 0.0029), and P-JNK expression levels were negatively correlated (r = -0.336, P = 0.034); P-JNK expression level and P-IκBα expression levels negatively correlated (r = -0.321, P = 0.043). Conclusions: 1. RhoGDIα the regulation of apoptosis in lung cancer cells, \lung cancer cell apoptosis; 3 low concentrations of paclitaxel (10nM)-induced lung cancer cells G2 / M phase arrest and apoptosis, while activation of ERK and NFκB pathway; 4. RhoGDIα downregulated genes can increase lung cancer cells to low concentrations of paclitaxel ( 10nM) drug sensitivity, and this effect is inhibited by activation of JNK pathway and NFκB, ERK pathway and BAD phosphorylation accomplished; 5 RhoGDIα protein expression in lung cancer was significantly increased, the expression level and P-JNK and P -BAD expression levels correlated; 6. binding in vitro cell research and tissue studies, suggesting RhoGDIα possible through JNK, BAD, NFκB and ERK pathway in lung cancer cell apoptosis and promote tumor development and progression.

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