Dissertation > Excellent graduate degree dissertation topics show

The Effect of Maternal Deprivation on the Behavior of Male Adult Rats and the Expression of Dopamine Transporter in Striatum

Author: MaXiuLing
Tutor: ZhuXiongZhao
School: Central South University
Course: Clinical Psychology
Keywords: maternal deprivation striatum dopamine transporter (DAT) DNA methylation
CLC: R741
Type: Master's thesis
Year: 2009
Downloads: 47
Quote: 0
Read: Download Dissertation

Abstract


ObjectiveTo observe the behavioral characterization of adult male rats after experiencing neonatal maternal deprivation and the effect of maternal deprivation on the expression of dopmine transporter (DAT) mRNA in adult male rats’ striatum and explore whether DNA methylation was involved in its expression regulatory mechanism.MethodsNewborn male rats were randomly divided into two groups, the maternal deprivation group (n=16) and the control group (n= 14).Beginning on post-natal days(PND) 1 through 14, each pup designated for deprivation was placed in an individual round plastic container with no bedding and remained isolated for 6 hours(9:00-15:00) per day in constant temperature (25℃),while the controls without any treatment. When both groups grew up to ten weeks old, their spatial learning and memory, exploratory ability in novel environments spontaneous and anxiety levels were assessed by Morris water maze,an open field test and an elevated plus maze. DAT mRNA expression level in striatum was detected by Reverse Transcription-PCR, and its DNA methylation levels were measured by bisulfite genomic sequencing PCR(BSP).ResultsTwo groups are significantly different in mean latency to reach the platform : maternal deprivation group(83.66±22.91s);control group(56.98±20.89s) in the Morris water maze experiment. (t=-2.39, p＜0.05) .The number crossing the paltform has significant difference between maternal deprivation group (2.56±1.55) and control group(4.21±1.58) (t=-2.89, p＜0.05) .The total time spent in the quadrant previously associated with the platform was significantly different:maternal deprivation group((25±9.52s);controlgroup(32,87±8.27s) (t=-2.39, p＜0.05) .Open-field test revealed that the exploratory ability decreased compared to the controls: maternal deprivation group(17.41±2.24m); control group(22.86±2.89m) (t=-5.81, p＜0.001) .The total time stayed in the centre squares was no great different: maternal deprivation group (13.13±5.22s) ; control group(9.42±5.16s) (t=-2.38, p＞0.05) .The elevated plus maze experiment showed that maternal deprivation group spent a greater amount of time in the open arms(u=2.35, p＜0.05).The number of entries into the open arms was not significant different (u=-0.99, p＞0.05) ;The number of entries into the closed arms Of maternal deprivation group was more than control group (u=2.351, p＜0.05) .The expression level of DATmRNA in the striatum of the maternal separation group (0.24±0.04) was significantly lower than that of the control group (0.48±0.11)(t=-4.76, P＜0.05). The DNA methylation levels in the promoter region of DAT were not significantly different between the maternal deprivation group (0.29±0.42) and the control group (0.29±0.20) (t=-0.18, P＞0.05).ConclusionAdult male rats that experienced neonatal maternal deprivation showed impairment in spatial learning and memory, exploratory ability in novel environments and spontaneous anxiety levels.We conclude that maternal deprivation could influence the expression of DAT mRNA in striatum. However, DNA methylation may not be involved.

Related Dissertations

  1. Studies on the Mechanism and Recovery Measures of Hyperhydricity of Pyrus Calleryana Decne in Vitro,S661.2
  2. Effects of 5-Aza-2-deoxycytidine on DNA Methylation and Expression of p16 and MGMT Gene in Lung Cancer Cell Line SPC-A-1,R734.2
  3. Methylation of p16 Gene in Plasma and Tissues from Non-small Cell Lung Cancer Patients, Demethylation and the Biological Behavior of Lung Cancer Cell and Transcription of p16 Gene,R734.2
  4. The Clinical Analysis of 13 Hemichorea Patients Caused by Acute Cerebaral Angiopathy,R743.3
  5. Thyroid cancer blood spectra of DNA methylation,R736.1
  6. The Effect of Chronic Intermittent Hypoxia on the Oxidative Stress in Corpus Striatum of Parkinson’s Disease Model Mouse,R742.5
  7. Basic Research of β1 Adrenergic Receptor Gene Methylation on the Different Expression and Correlation with Blood Pressure in Spontaneously Hypertensive Rats,R544.1
  8. The Significance of Promoter Hypermethylation of p16 Gene in Circulating DNA on Early Diagnosis of Non-small Cell Lung Cancer,R734.2
  9. Effects of Low Doses Levodopa Administration on Striatum Acetylcholine and M1、 M2 Receptors Expression in Parkinsonian Rats,R742.5
  10. Plasma DNA Analysis in Predicting Short-term Recurrence of Surgical Patients with Non-small Cell Lung Cancer,R734.2
  11. Effect of Chronic Ethanol Exposure and Withdrawal on the Synaptic Plasticity in Dorsal Striatum of Rat,R749.6
  12. Phthalates endocrine disrupting effects of chemicals,R131
  13. The Analysis of Molecular Markers and DNA Methylation in the Different Strains of Meretrix Meretrix,S917.4
  14. Motor function in age-related brain tissue structure of DTI studies,R87
  15. Investigate the Effects of DNA Methyl Transferases 1 on Proliferative of Human Umbilical Vein Smooth Muscle Cells Induced Byhomocysteic Acid,R346
  16. In Vitro Rapid Propagation and DNA Methylation Variation of Gardenia Jasminoides Eills,S567.19
  17. Analysis of DNA Methylation in Arabidopsis Thaliana Exposed to SO2,Q943.2
  18. The Genetic Analysis of Heat Tolerance and Genotypic Difference on Heat Shock Response in Cucumber,S642.2
  19. Effect of Drugs of Abuse on Uracil Release in Mice Striatum,R965
  20. Effect of Brain IL-1β in Hippocampal Neural Lesion Induced by Maternal Deprivation in Early Life,R741
  21. Sources of human embryonic striatum neural stem cells in vitro biological properties,Q813.1

CLC: > Medicine, health > Neurology and psychiatry > Neurology
© 2012 www.DissertationTopic.Net  Mobile