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The Effect of Drynaria Fortunei J.SM on the Expression of Osteoprotegerin and Serum Tartrate-resistant Acid Phosphatase5b in the OVX Rat

Author: ZhangYongJun
Tutor: ZhangDiHua
School: Central South University
Course: Surgery
Keywords: drynaria fortunei ovx rat osteoprotegerin artrate-resistant acid phosphatase5b
CLC: R580
Type: Master's thesis
Year: 2009
Downloads: 55
Quote: 1
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Objective:To investigate the effect of drynaria fortuei J.SM to the expression of the osteoprotegerin in the bone tissue and serum tartrate-resistant acid phosphatase5b of female ovx rats,which were cut the uterus and given the dosage of 15 weeks.Methods:With the help of relative reference,this experiment chose thirty female rats of 3 monthes which were cut uterus and the fat around the uterus in groups to give the dosage of 15 weeks.Then,kill the rat and get the lateralthigh’s bone tissue.Make it decalcification, dehydration and section,then stain it by osteoprotegerin immunohi -stochemistry.Observe it by the microscope and analysis by the soft of image on the computer to get the masc rate.Get the rat serum and determine the tartrate-resistant acid phosphatase5b by ELISA.Results:1.The osteoprotegerin masculine rate of experiment group and sham-operation group are obviously higher than that of contrast group p<0.05;2.The expression of serum tartrate-resistant acid phosphatase5b in experiment group and sham-operation group is obviously lower than contrast group p<0.05;3.The expression of osteoprotegerin and serum tartrate-resistant acid phosphatase5b between experiment group and sham-operation group showed no significant differences,not statistically significant P>0.05. Conclusion:Drynaria fortuei can promote the expression of osteoprotegerin in ovariectomized rats;Drynaria fortuei can inhibit the expression serum tartrate-resistant acid phosphatase5b in ovariectomized rats

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