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Effect of Quercetin or 17-AAG on Radiosensitivity of HeLa Cells and V79 Cells

Author: LiangXiaoFang
Tutor: ZhangBaoGuo
School: Suzhou University
Course: Radiation Medicine
Keywords: Quercetin 17-AAG Cell proliferation Radiosensitivity HeLa cells V79 cells
CLC: R563
Type: Master's thesis
Year: 2009
Downloads: 60
Quote: 0
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Abstract


Objective: study quercetin and 17-AAG on human cervical cancer cells HeLa and Chinese hamster lung fibroblast V79 cell proliferation inhibition and quercetin and 17-AAG combined X-ray of the radiosensitivity of HeLa cells and V79 cells the impact . Methods: MTT assay activity of different concentrations of quercetin and 17-AAG on cell proliferation of HeLa cells and V79 cells . The colony formation Determination cell survival rate , to study quercetin and 17-AAG on the radiosensitivity of HeLa cells and V79 cells . Results: 1 quercetin on human cervical cancer HeLa cells and Chinese hamster lung into fibers V79 cells proliferation was significantly inhibited , and there is a certain dose and time dependent relationship . Colony formation inhibition experiments showed obvious radiosensitizing effect of quercetin on HeLa cells and V79 . After drug treatment , the mean lethal dose (D0) of HeLa cells and V79 decreased , Dq value is less than the radiation alone group , the survival curve shoulder area smaller (Dq ) 150μmol / L , the concentration of quercetin , sensitizing than can approximate reach 2 and 1.5. 3 MTT assay showed 17-AAG in vitro inhibition of human cervical carcinoma HeLa cells and Chinese hamster lung into fibers V79 cells proliferation was observed , the study results show that HeLa cells significantly inhibited the role , and there exists a certain relationship, but dose and time dependent inhibition of V79 cells smaller . 4 smaller ( colony formation inhibition experiments radiosensitizing effect of 17-AAG in HeLa cells and HeLa cells after drug treatment , the mean lethal dose (D0) Dq value is less than the radiation alone group , the survival curve shoulder area Dq) ,17-AAG concentration 75nmol / L when sensitizing ratio can be approximated by up to 2.3 . 17-AAG radiosensitization of V79 cells had no significant effect . Conclusion : Quercetin can significantly inhibit the proliferation of HeLa cells and V79 cells and enhance their radiosensitivity ; 17-AAG significantly inhibited cell proliferation in HeLa cells and enhance their radiosensitivity radiosensitivity of V79 cells did not impact.

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