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Expression and Significance of Pokemon in Diffuse Large B-Cell Lymphoma and Multiple Myeloma

Author: LiangZuoQing
Tutor: WangLuQun
School: Shandong University
Course: Internal Medicine
Keywords: Pokemon Diffuse large B-cell lymphoma Multiple myeloma Immunohistochemistry RT-PCR Western blot
CLC: R733
Type: Master's thesis
Year: 2009
Downloads: 56
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Abstract


Objective: To study the Pokemon expression in diffuse large B-cell lymphoma (Diffuse Large B-cellLymphoma, DLBCL) and multiple myeloma (Multiple Myeloma, MM), explore the Pokemon role in the pathogenesis of DLBCL and MM possible molecular mechanisms. Research Methods: Using immunohistochemistry SP method detection Pokemon, p53, Bcl-6, CD10 and MUM1 protein in 50 patients with diffuse large B-cell lymphoma (DLBCL) and 10 cases of reactive hyperplasia lymph node (RLH) expression; using reverse transcriptase polymerase chain reaction (RT-PCR) and Western immunoblotting (Western Blot) detection Pokemon in human cervical carcinoma cell lines Hela, MM cell lines in KM3, 15 cases of multiple myeloma patients with bone marrow mononuclear cells (bone marrow blood mononuclear cells, BMMNCs) and 10 healthy human peripheral blood mononuclear cells (peripheral blood mononuclear cells, PBMNCs) expression. Results: 1.Pokemon protein expression in 50 cases of DLBCL and 10 cases of RLH were 35 cases (70.0%) and 1 (10.0%) was significantly higher than in the RLH expression (P Pokemon protein expression in DLBCL <0.01); Pokemon protein GCB (Germinal Center origin in 20 cases of B-cell) type (12/20, 60.0%) and 30 cases of non-GCB (non-Germinal Center origin of the B-cell) type DLBCL patients ( 23/30, 76.7%), the expression of the difference was not statistically significant (P> 0.05); expression of mutant p53 protein GCB type DLBCL (5/20, 25.0%) was significantly lower than in the non-GCB type DLBCL Expression (16/30, 53.3%) (P <0.05); 35 cases of Pokemon protein-positive DLBCL in 18 cases of mutant p53 protein, Bcl-6 protein was positive in 16 cases, and 15 cases of Pokemon protein negative DLBCL mutant p53 protein, Bcl-6 protein positive cases of DLBCL, Pokemon protein expression was positively correlated with mutant p53 protein and the expression of Bcl-6 protein. Pokemon protein expression was no significant difference (P> 0.05) in the DLBCL patients age and gender. GCB type DLBCL in 20 cases, nine cases occurred in the lymph nodes, accounting for 34.6% of the lymph nodes within the group (26 cases) occur in extranodal were 11 cases, accounting for 45.8% of the lymph nodes outside the group (24 cases), two distribution ratio the difference was not statistically significant (x ~ 2 = 0.654, P = 0.419). Pokemon protein expression in the lymph nodes, the outer group DLBCL difference was not statistically significant (x ~~ 2 = 1.847, P> 0.05). 2 by reverse transcription polymerase chain reaction (RT-PCR) and Western immunoblotting (Western Blot) detected Pokemon in human cervical carcinoma cell lines Hela, MM cell line KM3 showed over-expression in 15 cases of multiple myeloma tumor 6 patients with positive expression (6/15, 40%); patients and in only two cases showed low expression (2/10, 20%) in the peripheral blood of 10 healthy subjects. Conclusion: 1.50 cases of DLBCL Pokemon protein expression was 70.0%, 10 cases in the control group was 10.0%, there are significant differences between the two, Maeda et al. Detected 130 cases of DLBCL patients Pokemon protein expression rate agreement. Pokemon protein expression in GCB type and non-GCB type DLBCL rate of 60.0% and 76.7%, respectively Seen in this light, Pokemon protein expression in highly malignant non-GCB type rate than the GCB type has a higher trend but not statistically significant. This study examined the mutant p53 protein positive rate was 42.0% in DLBCL, significantly higher than the control group (0.0%), consistent with and domestic reports the results of the basic. Pokemon protein of positive expression and mutant p53 protein in the positive expression was being related, revealing Pokemon protein and mutant p53 protein co-participation in the DLBCL of the occurrence and development, the possible existence of Pokemon-p53 cell transformation pathway in the pathogenesis of DLBCL. Played an important role. 2.Pokemon over-expression in KM3 multiple myeloma cell lines by semi-quantitative analysis of four cases out of 15 cases of multiple myeloma patients strongly positive, 2 cases were positive, only two cases of 10 healthy subjects Pokemon weakly positive, preliminary evidence Pokemon expression in MM.

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CLC: > Medicine, health > Oncology > Hematopoietic and lymphoid neoplasms
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