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Diabetes is one of the major diseases of the threat to human life and health . Bitter gourd and isoflavones as traditional Chinese medicine to improve diabetes diet drugs , low toxicity , and a wide source , and thus carry out the bitter gourd the Pueraria Jiangtang granules research has important application value . Extraction, separation , and work on the chemical composition of the plant bitter melon and develop the quality standards of the bitter melon the Pueraria Jiangtang granules , while the hypoglycemic effect of bitter melon the Pueraria Jiangtang granules Granules preliminary animal experiments . The main results are as follows : (1) compare and choose the the bitter gourd traditional extraction method ( soaking method and reflux extraction ) , preliminary analysis of the solvent , temperature , time , and number of extraction reflux extraction effect . By reflux extraction technology optimization, to determine the appropriate extraction process : the reflux extracted three times with 75% alcohol as solvent , at a temperature of 80 ℃ under 8 hours at a time . This process for the extraction of similar plants later reference . On this basis , from bitter melon crude drug extract, separated by two single component . ( 2) by TLC puerarin qualitative identification , the results show that the law does not interfere with obvious spots , specificity , can be used as qualitative identification standards . Further by UV spectrophotometry , high performance liquid chromatography on the quality standards of the bitter gourd the Pueraria Jiangtang granules methodology . The results show that the method is accurate , high sensitivity , good reproducibility , and can be used for the determination of the ingredients in the granules quality standards . (3) The preliminary test results show that the the bitter gourd the Pueraria Jiangtang granules hypoglycemic effect the bitter gourd the Pueraria Jiangtang granules for STZ-induced hyperglycemic animal significantly reduce the effects of high blood sugar , and dose-response test for the next population a basis .
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