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Objective: serology and molecular biology techniques for slaughtering five hepatitis B and hepatitis B virus (HBV) DNA testing to determine the presence of viral infection, to understand the situation and pigs infected with HBV infection model to explore the pig as hepatitis B virus infection animal model is feasible. Methods: Blood collected slaughtering 5ml × 2 tubes, uniform numbers, a total of 283 specimens collected, a centrifugal separation of serum, 234 serum samples obtained, another tube anticoagulant anticoagulant, using traditional phenol-chloroform extraction leukocytes DNA, and the DNA extract 156. Enzyme-linked immunosorbent assay (ELISA) method for serum HBV markers (HBV maker, HBV-M, commonly known as \-DNA by polymerase chain reaction (PCR) to detect the HBV infection. Experiment with people's \Results: (1) 234 serum samples HBV markers test results: the measured serum markers of HBV-positive results by the common combinations of serum markers can be divided into eight modes: Combination Mode 1 (HBsAg-HBeAg and HBcAb ). Combination Mode 2 (HBsAg, HBeAb and HBcAb), combined mode 3 (HBsAg, HBcAb), combined mode 4 (HBsAb, HBeAb and HBcAb), combined mode 5 (HBsAb and HBcAb), combined mode 6 (HBsAb), combined Mode 7 (HBeAb and HBcAb-) combined mode 8 (HBcAb-) as a percentage was 0.43% (1/234), 0.85% (2/234), 2.99% (7/234), 0.43% (1 / 234), 26.07% (61/234), 2.99% (7/234), 15.38% (36/234), 34.19% (80/234). Serum markers of 39 wholly negative results, the percentage was 16.67% (39/234). (2) serum HBV-DNA by PCR amplification results: S District primers positive rate of 3.4% (8/234), C region primers positive rate of 20% (47/234). (3) leukocyte DNA extract HBV-DNA by PCR amplification results: S District primers positive rate of 10.9% (17/156), C region primers, the positive rate of 51.3% (80/156). (4) leukocyte detection rate of HBV-DNA in serum HBV-DNA detection rates, the difference was statistically significant (P lt; 0.001). (5) HBV-DNA detection rate in Area C of S region detection rate, the difference was statistically significant. Conclusions: (1) HBV in slaughter pigs have a higher prevalence of infection with human infection patterns similar pattern (2) WBC detection rate of HBV-DNA in serum HBV-DNA detection rate (3) C region of HBV-DNA detection test is more sensitive than the S region.
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