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Studies on Mechanism of Nitrogen and Phosphorus on Isoflavones Contents in Red Clover (Trifolium Pratense) and Anti-oxidant Performance of Isoflavones

Author: LuJuan
Tutor: DuWenHua
School: Gansu Agricultural University
Course: Grassland
Keywords: Minshan red clover Nitrogen and phosphorus fertilizer Isoflavone content Impact mechanism Antioxidant properties
CLC: S541.2
Type: Master's thesis
Year: 2009
Downloads: 62
Quote: 1
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Abstract


The test by studying fertilization mechanism of isoflavone content and isoflavone antioxidant properties, the following conclusions: 1, in all growth stages, ranging from four isoflavones content, but biochanin A ( Bio A) the highest content of thorns formononetin (For) second; in different parts of red clover isoflavone content there is a big difference, leaf content of the highest (8103.3ug / g), accounting for the total content of the whole plant isoflavones of 50%; flowers in the content, followed by (7130.1ug / g), 45%; stem lowest content (737.3ug / g), only 5% of the whole plant. Description red clover in the long development process, the different organs of metabolism of isoflavones there are tissue-specific distribution of isoflavones. 2, the Minshan red clover isoflavones in the total content in jointing and squaring period, and these two periods isoflavone content trend is not obvious. From the budding stage, the isoflavone content of a sharp rise in first flowering, full bloom, pod and maturity period showing high - low - high - low complex trend. Higher levels of the two periods of the first flowering and podding. 3 different growth stages, N, P fertilizer application rate, how many have a dramatic effect on the the Minshan red clover isoflavone content. With the increase in N, P fertilizer application rate, the Minshan red clover isoflavone content was reduced after the first increase trend, A3 (100 kg N / hm2), B2 (120kg P2O5/hm2) processing plant highest isoflavone content. PAL, SP, SS and MDA Minshan red clover isoflavone essential. PAL activity increased SP MDA content increased, leading to the increase in the number of synthetic isoflavone precursor of phenylalanine the adequate precursor substances stimulating isoflavones synthesized in large quantities, so that the different growth stages and different P fertilizer application rate under PAL SP MDA isoflavone content was significantly positively correlated; red clover growth and P fertilizer application rate the plants accumulate part of the sugar into the cell structure and storage material, the other part by the sugar metabolism - shikimate pathway final produce secondary metabolites such as amino acids or isoflavones, the SS content increased, reduced isoflavone content. Under different N fertilizer application rate PAL, SS, MDA isoflavone content was positively correlated with the SP and isoflavone content was negatively correlated. Different stages and different fertilization treatments SS and SP and isoflavone content inconsistent isoflavone mechanism needs further study. 5, the antioxidant properties of red clover isoflavones POD, CAT, SOD three enzyme activities greater. Under different N, P fertilizer application rate, POD, CAT, SOD activity and isoflavone content was negatively correlated. It is suggested that, the Minshan red clover in the antioxidant system is a unified whole, the accumulation of isoflavone content in the body to make up for the reduction of other antioxidant enzymes and antioxidants, so as to ensure the normal growth and development of plants. Plants at different growth stages, with the growth and development of the Minshan red clover, POD activity and isoflavone content of the first sharp decline in the budding stage and then begins to rise sharply, early flowering stage after three periods POD activity remained unchanged ; isoflavone content and POD activity was positively correlated. CAT activity in the plant was a \The \Isoflavone content and POD activity inconsistent in different stages and different fertilization treatments, the antioxidant properties of isoflavones for further study.

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CLC: > Agricultural Sciences > Crop > Fodder crops,pasture > Perennial legume > Clover
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