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Comparison of Tissue Angiotensin Ⅱ in Atherosclerotic between Proximal and Distal Sites of Aortic Constriction in Rabbit
Author: ZhaoSheng
Tutor: LiuFuYuan
School: Anhui Medical University,
Course: Internal Medicine
Keywords: High cholesterol Coarctation Artery Atherosclerosis Hypertension
CLC: R543.5
Type: Master's thesis
Year: 2009
Downloads: 28
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Abstract
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Objective: To investigate the changes in atherosclerotic rabbit abdominal aortic surgery after high-fat feeding narrowing the proximal and distal vascular smooth muscle cells and tissue angiotensin II (Ang II). METHODS: New Zealand white rabbits were randomly divided into normal group, high-fat group after 28 weeks of feeding, the two groups were randomly selected part of the abdominal aorta constriction, the rest of sham-operated. Thus divided into fat-operated group, high-fat sham operation group, operation group, sham-operated group. To continue to raise after 4 weeks, blood samples measured blood lipids. Obtain specimens measuring abdominal coarctation of the aorta proximal and distal vascular pressure. HE staining intimal media thickness, immunohistochemical analysis detected endometrial, film alpha-muscle actin and angiotensin II levels. Results: 1 high-fat group compared with the normal group, TC (23.03 ± 2.62:1.95 ± 0.14), TG (4.77 ± 0.81:1.48 ± 0.27), LDL (10.73 ± 1.12:1.75 ± 0.56) were significantly increased, p lt; 0.05; the ordinary surgery group and the fat surgery group blood vessels to narrow the proximal blood pressure than the remote, general surgery group (72.30 ± 13.40:54.10 ± 11.00), high-fat surgery group (84.18 ± 5.28:52.62 ± 5.90), p lt; 0.01; 3 fat hand group and high-fat sham operation group narrowing the film thickness in the proximal than the remote, high-fat sham operation group (154.04 ± 3.22:136.13 ± 4.30), fat operation group (174.42 ± 7.83:128.35 ± 8.13), p lt; 0.05, higher fat surgery group fat sham operation group narrow the proximal end of the film thickness increase (174.42 ± 7.83:154.04 ± 3.22), p lt; 0.05; 4 endometrial thickness: fat surgery group narrowed proximal than distal thickening (183.76 for ± 10.53:118.63 ± 13.35), p lt; 0.01; the fat surgery group constriction proximal higher fat the same location of the sham-operated group thickening (183.76 for ± 10.53:164.59 ± 4.96), p lt; 0.01; 5.α-actin levels: the intima, high fat surgery group narrowed proximal than narrowing the remote was significantly higher (0.13 ± 0.01: 0.08 ± 0.01), p lt; 0.01; the fat surgery group coarctation proximal higher fat sham operation group the narrow proximal significantly increased (0.13 ± 0.01:0.10 ± 0.02), p lt; 0.01; in the film, shrink The narrow proximal remote, but the surgery group (0.10 ± 0.03:0.08 ± 0.03), p gt; 0.05, fat-operated group (0.23 ± 0.04:0.15 ± 0.02) p lt; 0.05; fat surgery group reduction The narrow proximal higher fat sham group and the surgery group coarctation proximal pressure p lt; 0.05; 6.Ang level II: endometrial, high fat surgery group narrowed proximal than narrowing the remote was significantly higher ( 0.26 ± 0.01:0.12 ± 0.01), p lt; 0.01; higher fat surgery group narrowed proximal fat sham operation group was significantly higher (0.26 ± 0.01:0.17 ± 0.01), p lt; 0.01; in the film, shrink The narrow proximal remote, but the surgery group (0.11 ± 0.01:0.08 ± 0.02), p gt; 0.05, high-fat surgery group (0.28 ± 0.04:0.16 ± 0.02) p lt; 0.05; the fat surgery group shrink The narrow proximal higher fat sham group and operation group to narrow proximal elevated p lt; 0.05. Conclusion: simple rabbit abdominal aortic coarctation after 4 weeks, the narrow nearly caused - Remote pressure difference, can not lead to arterial intimal thickening the alpha actin protein, vascular tissue Ang Ⅱ. Only under the influence of high-fat high-pressure, blood vessel constriction proximal intima-media than the constrictive remote thickening tissue Ang II levels increased.
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CLC: > Medicine, health > Internal Medicine > Heart, blood vessels ( circulatory ) disease > Vascular disease > Artery disease
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