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This paper is divided into two parts, the first part of the determination of the different formulations of the same drug in the drug concentration in the aqueous humor, preliminary study of the absorption of different drug formulations differences; second part through breast milk drug analysis method, the rational use of drugs for scientific guidance lactating study basis. The first part LC-MS/MS method for the determination of cataract patients using gatifloxacin eye drops, gatifloxacin the gel Shaxing treatment of aqueous humor concentration of the drug, and thus provide a reference for eye preparations formulations. Aqueous humor samples after liquid-liquid extraction process, using the internal standard method (internal standard ciprofloxacin) for quantitative analysis. Liquid phase conditions: Column: ZORBAX Eclipse Plus C18 (2.1 × 100mm, 1.8μm); mobile phase: 0.1% formic acid: acetonitrile (0.1% formic acid) = 80:20 (V / V); Flow rate: 0.3mL/min ; column temperature: 30 ° C; injection volume: 5 μL. MS conditions: ESI ion source, the positive ion mode, select the MRM mode tandem mass spectrometry analysis. Gatifloxacin (m / z): 376.1 → 332.1, the peak time 7.1min: ciprofloxacin internal standard (m / z): 332.1 → 288.1 peak time 7.5min. Aqueous humor of endogenous substances do not interfere with the Gatifloxacin determination. Aqueous humor samples were divided into two groups according to the experimental requirements. Low concentrations of gatifloxacin 0.5 ~ 12.0ng · mL -1 was a good linear relationship, linear regression equation Y = 1.9291X .6016 (r = 0.9964), when gatifloxacin star concentration as 1.0ng/mL, 6.0ng/mL when 40.0ng/mL of recoveries were 114.1%, 106.5%, 83.2%, RSD were 20.3%, 9.1%, 9.5% (n = 5) were determination requirements; biological samples high concentration group, and gatifloxacin in of 10.0 ~ 200.0ng · mL -1 showed a good linear relationship between the linear regression equation Y = 1.2665X 3.5886 (r = 0.9983), plus gatifloxacin concentration 1.0ng/mL, 12.0ng/mL, 150.0ng/mL recoveries were 114.1%, 101.1%, 102.0%, RSD were 20.3%, 8.4%, 5.6% (n = 5), are in line with the determination of the biological sample requirements. Will establish the analytical method used in 34 cases of use of eye drops in patients and 40 patients gels, the test showed that the great RSD of 74 cases of patients aqueous humor sample measurement results can be clearly observed individual differences phenomenon. Will use the drug concentration of the two formulations in aqueous humor after the results of statistical analysis, showed that gatifloxacin gatifloxacin gel gatifloxacin eye drops compared to aqueous humor drug concentration was significantly higher gatifloxacin eye gel drug concentration in the aqueous humor can maintain a higher level, with better clinical efficacy; an hour or so after treatment, the drug concentration of the aqueous humor reaches the maximum, it is recommended that the medication about one hour before surgery, the suppression bacteria best. The second part, determined by HPLC lactation mastitis were treated with the drug concentration in the milk after treatment of cefuroxime sodium. Defatted milk samples, protein precipitation, the use of the liquid phase Agilent ZORBAX SB-C18 column (4.6mm × 150mm, 5μm), sodium acetate buffer (pH3.7): acetonitrile = 7:1 as the mobile phase flow rate was 1.0mL · min -1 , the column temperature was maintained at 30 ° C, detection wavelength 254nm, milk drug concentration determination. The results showed that the milk endogenous substances do not interfere with the sample measured cefuroxime sodium in the 0.02 to 10 μg of · mL -1 concentration range showed a good linear relationship, the regression equation was Y = 16872X-273.38 ( r = 0.9980), the limit of quantification was 0.02 μg · mL -1 . High, medium, low concentrations of recoveries were 110.2%, 93.2%, 102.0%, RSD were 4.6%, 3.2%, 9.1%, in line with recovery and precision determination of requirements for biological samples. This topic determined by HPLC lactation mastitis were treated with the drug concentration in the milk after levofloxacin treatment. Defatted milk samples, protein precipitation using Agilent ZORBAX SB-C18 HPLC Columns (4.6mm × 150mm, 5μm), citric acid solution (0.05mol · L -1 , triacetate amine adjust pH 3.4): acetonitrile = 85:15 as mobile phase, a flow rate of 1.0 mL · min -1 , the column temperature was 30 ° C, detection wavelength 293nm milk drug concentrations were measured. The results showed that the milk of endogenous substances do not interfere with the sample determination, levofloxacin 0.03 ~ 15.5μg · mL -1 concentration range was a good linear relationship, the regression equation is Y =, 48472X 4155.2 (r = 0.9988 ), the lower limit of quantification 0.03μg · mL -1 . High, medium, low concentrations of recoveries were 108.6%, 107.2%, 109.1%, RSD were 4.5%, 4.7%, 5.5%, in line with recovery and precision determination of requirements for biological samples. Currently, cefuroxime sodium, levofloxacin drugs commonly used in patients with mastitis. Milk drug concentration determination method, the elimination can examine the metabolism of the drug in the milk, improve the security of breastfeeding foundation.
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