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Cranberry Ericaceae Vaccinium (Ericaceae) (Vaccinium. SPP) perennial deciduous or evergreen shrubs, distributed in Europe, Russia, North America, the Alps and the Chinese parts. Worldwide, there are more than 400 cultivars. Vaccinium species in China is located in East China, Central China, South China, to the southwest and northeast regions, more common in the hills and 400-1400 meters above sea level areas. Bilberry fruit blue-purple, delicate flesh, small seeds, moderately sweet and sour, with a pleasant aroma. Fresh, processed into juice drinks, wine drinks. The unique fruit blueberry anthocyanins and other substances to improve vision, anti-aging and anti-cancer effect, known as \one of the five major human health food in recent years, popular in Europe, America and Japan. However, due to the summer in summer, and cranberry's shallow root system and drought resistance, resulting in a narrow cranberry cultivation area in Chongqing. One of the effective ways to change this situation, that is, the method of combining application of mutation breeding and conventional breeding methods and modern molecular biology techniques, namely, artificial induced genetic variation screening and identification of multiple generations, the development of high yielding quality and anti- strong new varieties of cranberries. Currently, more concentrated cranberries somaclonal variation research on tissue culture conditions and screening of pharmaceutical formulations plant screening variation in tissue culture process. But the role of the mutagen organism, the genetic material within the cell on the structure and function will often change, and be able to change the genetic trait of the plant, so that the DNA molecule damage, so that it changes in the replication process , caused by the mutation. Chemical mutagens, can be induced by the high frequency of clonal variation, some excellent of beneficial mutations traits and traits mutant selection pressure, can be screened. This test uses a combination of chemical mutagenesis and somaclonal variation induced and screening cranberries drought-resistant mutants, a preliminary study and treatment plants, generally can be divided into three parts: 1. Established blueberry regeneration system of cranberries chemical mutagen treatment, the regenerated plant height, leaf length, leaf width, mutation rate, rooting rate statistics. The results show that: the cranberries shoot tip in by 0.1% --- 0.6% EMS the plantlets mortality is zero; concentration of 0.3% EMS for optimal concentration of material V3 and 3 # plantlets average strain high reached 4.74cm and 4.52cm, average leaf length and leaf width multiplied average 1.173cm2 and 1.073cm2 When the the EMS concentration is 0.6%, the growth potential weak, narrow in the upper part and yellow-green, leaf loss. A preliminary study of regenerated plants obtained after processing, before and after drought treatment by measuring physiological indicators associated with drought tolerance, and filter out may occur drought-resistant mutants of EMS concentration. The results show that: (1) under drought stress, CK MDA content increased with the duration of stress time greatest; contrast, 0.1% and 0.5% for the MDA content than only a small increase in the pre-treatment difference not significant. (2) The EMS treatment plant regeneration soluble sugar content were higher than that of the control material V3 # soluble sugar content of 0.3% EMS treatment were increased by 107.3% and 83.7%, a significant difference. (3) material V3 and 3 # M0 generation by 0.3% EMS treatment plants of SOD, POD activity is still significantly higher than the control, an increase of 2.09% and 35.97%, respectively; 188.73% and 142.8%, respectively, with a significant difference. (4) For the activity of CAT activity, material V3 by 0.6% EMS treatment were increased by 84.6%, its activity is lower than that of the control after 0.2% and 0.5% EMS treatment; materials # 0.1% and 0.6% EMS its activity after treatment increased by 48.72% and 62.15%, respectively, compared with the control. M0 generation plants after drought stress after the initial screening, hydrogen peroxide enzyme (CAT), superoxide dismutase (SOD), peroxidase (POD) activity increased, malondialdehyde (MDA), reduced to drought environment showed some resistance. These two groups of materials has been a concentration of 0.3% EMS mutagenic treatment may exist after the drought resistance of the mutant plants. 3. Cranberries phenotypic screening of mutant plants. Mutagen treatment of 460 M0 plants were screened out of the 9 monoclonal obvious phenotypic variation, mainly of whole leaf morphology and leaf color variation. Mutagenesis phenotypic variation frequency is very low, only 1.96%. The variant plants by leaf thickness, the diameter of the main vein, palisade tissue thickness and leaf tissue structure tightness 9 associated with drought leaf anatomical structure indexes single factor multiple comparisons, selected four of the most sensitive indicators of membership function analysis : stomatal density, leaf thickness, the thickness of the upper epidermis, palisade tissue thickness. Application membership function of resistance to the high sensitivity of the indicators of the variant plants to filter out a comprehensive evaluation of drought resistance. The results showed that: the 9 variation cranberries plants and control materials drought resistance as follows: V-5 gt; V-9 gt; V-4 gt; V-7 gt; V-3 gt; V-6 gt; CK gt ; V-1 gt; V-2 gt; V-8.
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