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Analysis on Genetic Variation and DNA Methylation of Different Loquat Ploidies

Author: BianYu
Tutor: LiangGuoLu；WangWeiXing
School: Southwestern University
Course: Cell Biology
Keywords: Loquat Polyploid Genomic variation DNA methylation
CLC: S667.3
Type: Master's thesis
Year: 2010
Downloads: 207
Quote: 2
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Abstract

Polyploidy phenomena play an important role in the evolution of higher plants, plant polyploidy derived from a large number of repeat sequences, they are beneficial resources of gene selection, evolution and mutation, loss and insertion of a large number of sequences, but also for plant evolution more choices. The polyploid attributed to the widespread presence of polyploid species and their diploid ancestors compared with adaptation for survival potential broader living environment and unstable weather conditions, such as polyploid important species forming process having selective advantage. The use of this test amplified fragment length polymorphism (Amplified fragment length polymorphism, AFLP) and methylation sensitive amplified polymorphism (Methylation sensitive amplified Polymorphism, MSAP), 'Luzhou No. 6', 'Longquan' and 'Early Red' three loquat times polyploid genetic differences between DNA methylation level and pattern differences provide a theoretical basis for the development and utilization of the loquat different ploidy material and ploidy breeding process the loquat even plant polyploidy and Genetic variation research has important reference value, the test results are as follows: AFLP analysis of the test results showed that different ploidy 'Luzhou' loquat polyploid than diploid genome a significant change in the total mutation rate between 6% -6.5%, with a rate of increase of 2.8% to 3.3%, with a loss rate of between 3% -3.3%; compared with diploid 'Longquan 1 No. 'loquat triploid and tetraploid total genomic mutation rate of 11% and 10.9%, respectively, strip the rate of increase of 7.2% and 5.7%, respectively, with a loss rate of 3.8% and 5.2%, respectively. 'Early Red' loquat triploid and tetraploid than diploid genome mutation rate of 12.1% and 11%, respectively, strip the rate of increase of 6.9% and 6.2%, respectively, the absent band of 5.1% and 4.8%. Three loquat cultivars polyploid polyploidy genome of the variation. MSAP analysis of test results show that 'Luzhou' triploid loquat genomic methylation was 12.8%, higher than the diploid, 'Luzhou the' tetraploid Lee pentaploids loquat the total methylation rates were 11% and 12%, were lower than the diploid; triploid and tetraploid methylation rates were 8.5% and 8.1%, 9.1% lower than the diploid and pentaploid full methylation rate of 9.2%, higher than the diploid. 'Longquan No.1' the triploid total methylation was 16.2%, higher than the 14.4% of the tetraploid, 11.4% higher than the diploid; 'Longquan' triploid loquat full methylation rate (10.3%) higher than tetraploid (9.5%) higher than the diploid (6.8%). 'Early Red 3' the loquat triploid total methylation was 15.3%, higher than the 13.1% of the tetraploid and diploid 12.2%; triploid full methylation highest rate of 11.6% , higher than the diploid (8.5%) the tetraploid methylation rate (7.9%) lower than the diploid. 'Luzhou', 'Longquan No. 1', 'early red 3' three loquat varieties found when comparing them of different ploidy polyploid loquat methylation level, three species of triploid loquat total methylation levels were higher than the same variety of other ploidy, tetraploid or five times the total methylation level or higher than the diploid or lower than the diploid, did not exhibit increased with the times increase or decrease in the methylation level of ploidy effects. The consolidated results of this study that the process of loquat times a certain degree of genomic DNA sequence variation and methylation level and pattern of inheritance changed, and there are sequence differences between different polyploid; speculate loquat triploid hybrids advantage of the formation of genomic variation and DNA methylation patterns of re-adjustment, especially a large number of over-or hypermethylation variation, but chromosome doubling and DNA methylation patterns to adjust the degree of polyploid traits and phenotypic changes have a certain affected.

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