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Konjac is Monocotyledoneae (Monocotyledoeae) Araceae (Araceae) Amorphophallus (Amorphophallus Blume) perennial herb, mainly in Asia, India, Indochina and Yunnan-based China Southern and Southeast Asia. China has discovered and named 21 species endemic to China nine kinds, mainly in the provinces south of the Qinling mountains and plateaus. This study has collected various domestic konjac germplasm resources were finishing, character classification, morphological identification and ISSR study to examine their genetic relationship to clarify their taxonomic status, and on the basis of preliminary develop a basic description of the plant Amorphophallus norms and terminology. Konjac germplasm resources for our preservation, protection and utilization provide a theoretical basis. The main results are as follows: 1, the first collection of domestic and foreign resources to organize 79 konjac, character classification, through character identification, have been identified Amorphophallus resources 64, unidentified material out there 15. Using morphological identification method of test material for the test to analyze the morphological traits Q-type cluster analysis, to facilitate the analysis, in the forest level with lines marked in the figure, when the level of bonding wires L (D = 11.00) to 79 konjac material is divided into six categories. 2, konjac germplasm by ISSR reaction system to optimize the analysis, and the temperature of each primer withdraws and PCR amplification cycles gradient screening, and ultimately establish a suitable konjac plant ISSR reaction system and PCR amplification parameters: In 25ulISSR amplification reaction system containing 1ul of template DNA, 200 umol / L of dNTP, 1.5U/25ul the TapDNA polymerase, 0.5umol / L primer, 2.5ulMgCl 2 , 2.5u110 × buffer buffer and ddH 2 O16.7ul. PCR amplification program: 94 ° C denaturation for 5min, 94 ° C denaturation 1min ,50-55 ° C (annealing temperature of each primer different) annealing 1min, 72 ° C refolding 70s, 40 cycles; final extension of 72 ° C 10min, 4 ° C preservation. Using the optimized reaction system of 49 parts of konjac germplasm ISSR-PCR amplification, a variety of qualitative similarity between the coefficients ranged between 0.68-1.00, indicating that the genetic differences between the materials konjac little. When the level of genetic similarity take D = 0.80 when tested in 49 konjac materials significantly clustered into five categories. 3, through dialogue konjac, konjac, West Union konjac, konjac and materials 64 bulbils bulbs moisture, glucomannan content, flesh color, texture and konjac corms profile Determination of color film after drying and observation, draw white Amorphophallus Amorphophallus konjac and high water content, glucomannan content, flesh color is white, bulbous profile finer texture and color after drying konjac piece is relatively good, so the white flower Amorphophallus konjac and integrated best quality; West League konjac followed; comprehensive quality material 64 worst. 4, for the first time in detail the four flower Amorphophallus observation and analysis, the earliest flowering Amorphophallus konjac, short flowering period, Yunnan Amorphophallus konjac and white, followed by West Union konjac latest, longest flowering. By konjac odor adnexal findings, Yunnan konjac adnexal flavor and odor to attract pollinating bees. By Amorphophallus konjac flower through a comparative analysis of morphological traits showed that the shape of spathe, spadix / spathe, adnexal shape, with or without the sexual organs, appendages odor and color, such as several mature fruit morphological traits large differences between species; Arum Characters in intraspecific more stable performance, only peduncle color and markings and appendages have some differences in color. 5, referring to China's existing resources Characters konjac, combined with the resources that have been collected traits observed for the first time initially established a basic description of konjac resources norms and terminology; and resources for the development of konjac DUS testing guidelines provide a theoretical basis. Konjac Germplasm test traits total 33, can be divided into traits and secondary traits will be measured, which will be measured traits 14, supplementary characters 19
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