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Cardioprotective Effects of Bicyclol Against Ischemia-reperfusion Injury in Rat

Author: CuiJie
Tutor: XiaQiang
School: Zhejiang University
Course: Physiology
Keywords: Bicyclol Myocardial ischemia and reperfusion injury Antioxidant Mitochondrial permeability transition pore
CLC: R965
Type: Master's thesis
Year: 2010
Downloads: 33
Quote: 0
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Abstract


Purpose : use of rat myocardial ischemia reperfusion model , explore bicyclol against ischemia reperfusion induced myocardial injury and its mechanism of action . Methods: SD male rats at 50, 100, 200 mg / kg / d bicyclol gavage three days , the last dose 6 h after ligation of the left anterior descending artery 1 h , 3 h simulated reperfusion myocardial ischemia and reperfusion model . Determination of myocardial infarct size by TTC staining . Simultaneous determination of serum lactate dehydrogenase ( lactate dehydrogenase , LDH ) content , the myocardial tissues superoxide dismutase (superoxide dismutase, SOD) activity , myocardial tissue inhibit the ability of hydroxyl radicals . Rat cardiac myocytes were enzymatically dissociated preparation cardiomyocytes during hypoxia and reoxygenation model , namely hypoxia 30 min, the Reoxygenation 60 min . Cell activity was measured using trypan blue . Determination of ROS (reactive oxygen species, ROS) generation and mitochondrial membrane potential fluorescent probe DCFH-DA and TMRE . And measured at 520 nm by 200μM CaCl 2 caused the opening of the mitochondrial permeability transition pore . Results: bicyclol significantly improved body ischemia reperfusion during cardiac hemodynamics , increased activity after myocardial cells hypoxia reoxygenation reduce myocardial infarct size and serum LDH levels after ischemia and reperfusion . Bicyclol can significantly improve the myocardial tissue inhibitor of hydroxyl radical generation capacity and SOD activity of scavenging oxygen free radicals , inhibiting the production of ROS myocardial cells after hypoxia reoxygenation . Bicyclic alcohol inhibition caused by hypoxia and reoxygenation cardiomyocytes DCF and TMRE fluorescence intensity increase and the absorbance values ??at 520 nm by CaCl 2 induced mitochondrial decline . Conclusion: bicyclol can against myocardial ischemia reperfusion injury , such myocardial protective effect may be achieved by inhibiting the generation of reactive oxygen species and mitochondrial permeability transition pore opening .

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