Dissertation > Excellent graduate degree dissertation topics show

Effects of Shrna-VEGF Interference on Cell Growth and VEGF Expression of Tongue Cancer Tca/DDP Cell Line

Author: HuangYan
Tutor: NongXiaoLin
School: Guangxi Medical University
Course: Oral and Maxillofacial Surgery
Keywords: short hairpin RNA human tongue squamous cell carcinoma drug-resistance cell lines vascular endothelial growth factor
CLC: R739.8
Type: Master's thesis
Year: 2010
Downloads: 26
Quote: 1
Read: Download Dissertation

Abstract


Objective:(1)To explore the mRNA and protein expression of vascular endothelial growth factor (VEGF) in human tongue squamous cell carcinoma cell line(Tca8113)and its Cisplatin-induced drug-resistant cell line (Tca/DDP). (2)To transfect short-hairpin RNA(shRNA)eukaryotic expression vector targeting VEGF constructed by the previous experiment into Tca/DDP cells cultured in vitro, as well as to observe the inhibition of VEGF expression and proliferation of Tca/DDP cells.Methods:(1)MTT assay was used to test the sensitivity to different chemotherapeutic medicines of Tca/DDP cells and parental cells;cellular immunohistochemistry was applied to detect multi-drug resistant protein P-gp expression in both parent cells and Tca/DDP cells. (2)Reverse transcription-polymerase chain reaction (RT-PCR) and Western Blot were applied to detect the mRNA and protein of VEGF in Tca8113 cells and Tca/DDP cells cultured in vitro.(3)The VEGF-targeting eukaryotic expression vectors were transfected into Tca/DDP cells by liposome LipofectamineTM 2000. RT-PCR and Western Blot were performed to analyze the inhibition of VEGF expression 48 hours after the transfection mediated by LipofectamineTM 2000. (4) MTT assay was used to explore the effect of interference on Tca/DDP cell growth. Results:(1)MTT assay showed that the tolerance of Cisplatin-induced drug-resisdant cells to different chemotherapeutic drugs had been increased to different extents;expression of P-gp was expressed higher in Cisplatin-resistant cells.(2) The expression of VEGF mRNA and protein in Tca/DDP cells was not significantly different from that in Tca8113 cells.(3)Forty-eight hours after the transfection mediated by LipofectamineTM 2000, RT-PCR and Western Blot showed the mRNA and protein of VEGF in Tca3/DDP cells significantly decreased in the interference group, compared by the non-transfected group, mock control group and scrambled sequence group.(4) MTT assay showed that the growth of Tca/DDP cells of interference group were decreased significantly through inhibiting VEGF expression, compared with that of the non-transfected group, mock control group and scrambled sequence groups 24 hours,48 hours and 72 hours after transfection.Conclusion:(1)Suggesting that Tca/DDP cells have the biological properties of multi-drug resistance.(2) VEGF expression exsisted and had no significant difference in Tca/DDP and Tca8113 cells. (3)The shRNA targeting VEGF, constructed with pGenesil1.1 vector, can inhibit the mRNA and protein expression of VEGF in Cisplatin-induced drug-resistant tongue cancer cells. (4)Targeting of VEGF can decrease the growth of Tca/DDP cells suggesting that targeting inhibition of VEGF gene may lay some theoretical foundation for the research into treatment of cisplatin-resistant tongue squamous cell carcinoma.

Related Dissertations

  1. Design, Synthesis and Biological Evaluation of Novel Podophyllotoxin Derivatives as Antitumor Agents,R284
  2. The Correlation Between Drug Resistance and Some Drug Resistance Genes in Sixty-one Gallibacterium Anatis Isolates,S852.61
  3. Inhibition of Myostatin (MSTN) Expresstion in Primary Porcine Fetal Fibroblasts by Lentivector-mediated RNAi,S828
  4. The Expression of MMP-2、 VEGF and Ki-67 and Human Glioma Grade,R739.41
  5. The Research on Treatment of Lentivirus-mediated hVEGF-165 Gene Transferring Mesenchymal Stem Cells on Rat Hind Limb Ischemia,R329
  6. Clinical Research of Bacteria Spectrum and the Change of Bacterial Drug Resistance About Pediatric Diseases During Six Years,R446.5
  7. BMP2 and VEGF165 Genes Transfected Rat Bone Marrow Mesechymal Stem Cells Simultaneity Expression,R329
  8. Effect of Proteasome Inhibitor Bortezomib on MDR1 Gene Expression in K562 Cell,R733.7
  9. Expression of TNF-α, MMP-9 and VEGF in BALF of Rats with Emphysema and Their Mutual Relationship,R563.3
  10. Influence and Significance of MMP-2 and VEGF of Hemangioma Endothelial Cells in Vitro Culture by Propranolol,R739.5
  11. Effect of the Function of MRP1 on Human Lung Adenocarcinoma Chemotherapy Resistance,R734.2
  12. Study of the Reversal Effect of Recombinant Human Endostatin on Chemotherapy Resistance in Human Lung Adenocarcinoma A549/DDP Cells,R734.2
  13. The Investigation of Relationship between Therapeutic Effect and Prognosis and Changes of Peripheral Blood Vascular Endothelial Growth Factor and Metallothionein in Patients with Advanced Lung Cancer,R734.2
  14. Study on the Correlation btween Serum, Aqueous Humor VEGF, IL-6 Levels and the Neovascular Glaucoma,R775
  15. The Analysis of the Growth Factor Related with the Secondary Glaucoma in the Anterior Champer of Silicone Oil-filled Eyes,R779.6
  16. Vascular endothelial growth factor on endothelial cells concentration of magnesium ions,R965
  17. Experimental Studies on Therapy of Lung Cancer by VEGF-C siRNA Mediated by Liposomes,R734.2
  18. Lesion Bacterial Investigation of Patients Suffer from Cutaneous Squamous Cell Carcinoma,R739.5
  19. Expression of RRM1 and MDR1 in Cervical Squamous Cell Carcinoma and Its Relationship with the Chemo-sensitivity to NACT,R737.33
  20. Meningiomas and MMP-9, VEGF expression and tumor angiogenesis and recurrence,R739.45
  21. Vascular Endothelial Growth Factor Gene Transfection Bone Marrow Stromal Cells Establish Scaffold to Treat the Ischemic Necrosis of Lunate Bone of Dogs,S858.292

CLC: > Medicine, health > Oncology > Oral cavity, maxillofacial tumors
© 2012 www.DissertationTopic.Net  Mobile