Dissertation > Excellent graduate degree dissertation topics show

Immunohistochemical Analyses of Changes in the Protein Expression of PKA-α and PKA-β as Well as Tau and PHF-1 in Four Brain Areas of Adult Female Rats with Heroin-Addiction

Author: HuHua
Tutor: YeJun
School: Guangxi Medical University
Course: Human Anatomy and Embryology
Keywords: Heroin addiction Nucleus accumbens Hippocampus Amygdaloid nuclei cAMP-dependent protein kin ase-α cAMP- dependent protein kinaseS Tau(S396) Paried helical filaments-1
CLC: R749.64
Type: Master's thesis
Year: 2010
Downloads: 56
Quote: 0
Read: Download Dissertation

Abstract


Objective To mimic the clinical patterns for human heroin- abusers, the animal models of heroin addiction were established so as to explore the changes of the PKA-α(cAMP-dependent protein kinase-α)and PKA-β(cAMP- dependent protein kinase-β) as well as Tau(S396) and PHF-1(paried helical filaments-1)in the cortex of frontal lobes and nucleus accumbens as well as hippocampus and amygdaloid nuclei. Method By using of gradual increase of heroin dosages, adult female Sprague - Dawley rats were given subcutaneous injections of heroin and the rat models with heroin addiction were made. The protein expressions patterns of PKA-α, PKA-β, Tau(S396)and PHF-1 in the cortex of frontal lobes, nucleus accumbens, hippocampus, and amygdaloid nuclei of the heroin- addicted rats were detected by utilizng immunohistochemistry. Result The numbers of the positive cells of PKA-αand PK A-βas well as Tau(S396) and PHF-1 in the cortex of frontal lobe, nucleus accumbens, hippocampus, and amygdaloid nuclei of the heroin– addicted rats were significantly higher than those of the control group rats (P<0.01). Conclusion The increasing amount of PKA-αand PKA -β, the overphosphorylation in the 396 suits of Tau protein and the formation of PHFs commonly exist in the cells of the above four brain regions, indicating that these abnormal changes may be a neuropathologic basis for showing the clinical symptoms of long heroin abusers.

Related Dissertations

  1. Based on statistical methods of magnetic resonance imaging of the human brain image segmentation and three-dimensional analysis of the data,R445.2
  2. Apolipoprotein E Gene Knock-out and High-fat Diet on IP3、 IP3R-1 ＆ Aβ Expression in Neurons of Mice’s Hippocampal CA1 ＆ Ca3,R329
  3. Effects of Selenium on Synaptosome Membrane Fluidity and PSD-93 of Hippocampus in Rats with Drinking Water Flurosis,R599
  4. The Molecular Mechanism Intervention of Selenium on the Learning and Memory Injury by Drinking Water Fluorosis,R599
  5. Effects of Partial Hepatic Ischemia/Reperfusion Injury on Postoperative Cognitive Function in Mice,R614
  6. Intranasal Delivery of Transforming Growth Factor-beta1 Is Neuroprotective Against Hippocampal Damage After Lithium-pilocarpine Induced Status Epilepticus,R742.1
  7. Tissue-Specific Expression and the Regulation of Porcine FTO,S828
  8. Effect of Curcumin on Hippocampal No-Nos System of Growing Pigs Subjected to Transport Stress,S858.28
  9. The Role and Mechanism of Hippocampus H2S/CBS Systemon Epilepsy Production,R742.1
  10. Gulf War syndrome in rats hippocampal neuronal apoptosis and Omi/HtrA2, Smac expression,R82
  11. Effects of Madecassoside on Learning and Memory of Chronic Aluminum Exposed Rats,R285.5
  12. Effect of Chronic Intermittent Hypoxia on the Level of Autophagy in Mice Hippocampal CA1 Neurons,R766.43
  13. The Influence of the Speed of Rising of Blood Glucose in Rats with Brain Damage Caused by Hypoglycemia,R587.2
  14. Chronic Stress Impairs Emotion and Cognitive Performance, Down-regulates Expression of FGF2 and FGFR1 Protein in Hippocampus and Prefrontal Cortex of Rats,R749.9
  15. Ghrelin every role of nuclear regulation in the hippocampus in learning and memory,R363
  16. Preliminary Study of MicroRNA Expression in Hippocampus of Rats with Cognitive Disorders After Traumatic Brain Injury,R651.15
  17. Effects of Melatonin on TGF-β3 Level in Hippocampus of Rats with Seizures Induced by Kainic Acid,R742.1
  18. Effects of Rem Sleep Deprivation on CRF and CRF Receptors mRNA in Depressive Rats’ Medulla Oblongata、hippocampus and Hypothalamus Treated with Chronic Stress,R749.4
  19. Rat medial septal nucleus injection of amyloid beta protein affect hippocampal theta rhythm and long-term potentiation in vivo electrophysiological study,R749.16
  20. Neurotoxicity of Amyloid β-protein and Its Possible α7 Nachr Mechanism: a in Vivo Electrophysiological and Behavioral Study in Rats,R749.16
  21. Fluoxetine for depression after stroke hippocampal tissue factor intervention,R749.1

CLC: > Medicine, health > Neurology and psychiatry > Psychiatry > Mental disorders of toxic
© 2012 www.DissertationTopic.Net  Mobile