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Background: Bladder cancer is a major public issue in the United States, bladder cancer is the fourth high incidence of malignancy, diagnostic column bladder cancer in patients with long-term survival rate is only 6%. Bladder cancer is the most common malignancy of the urinary system in China, seriously endangering the health of the people. The report shows that some cities in China in recent years, tumor incidence tended to increase the incidence of bladder cancer. Although chemotherapy for the treatment of advanced bladder cancer has improved significantly, due to early recurrence of the tumor, the prognosis is still very poor, and at the same time because the course of bladder cancer is often long and requires intermittent cystoscopy, urine cytology and radiological follow-up examination, making it one of the United States the cost of treatment is the most expensive of the tumor. Therefore, a new method to explore the effective treatment of bladder cancer has become clinically urgent need to address the actual problem. TGF-β (transforming growth factorbeta, transforming growth factor-β) is a multifunctional polypeptide cytokines, play an important role in the regulation of cell growth and differentiation, the high levels of TGF-β to promote angiogenesis, tumor cells invasion, metastasis, and immunosuppression, a large number of studies have found significantly higher in the liver, stomach, adrenal cancer, and bladder cancer cells and / or plasma of patients with TGF-β1 expression levels by inhibiting T-cell tumors produce high levels of TGF-β1 activating factor in the generation of IL-2 activity and inhibition of T cell proliferation, TGF-β can inhibit the growth and activity of the peripheral lymph nodes dendritic cells (DCs), but also directly increase tumor cell invasion, proliferative capacity, so the TGF- β is a tumor, the development process is important promoting factor. Matrix metalloproteinases (matrix metalloproteinases, MMPs) are a group structure has significant homology, zinc-dependent degradation of extracellular matrix peptidase general term, at least 26. Previous studies have shown the abnormal expression of MMPs may be associated with the occurrence of tumor invasion and metastasis, MMP2, abnormal expression of MMP9 in malignant most obvious, studies have shown that TGF-β1 affect tumorigenesis through regulation of MMPs, development. Integrin family (integrins) is a cell adhesion molecule, it is constituted by two transmembrane protein subunits (α, β), in a mammal, a total of 18 α-subunit and 8 β-subunit, they may be formed by combining 24 kinds of the disintegrin protein molecules, with different cell - extracellular matrix molecules (ECM) combining the regulation of cell adhesion, migration ability. The latest study found to play an important role in integrin β1 (integrin-β1) subfamily in the regulation of human tumor cell migration, invasion, metastasis, and high expression of integrin-β1 invasive bladder cancer. The study found that tumor tissue increased TGF-β1 expression will affect integrinα5, αv subunit and integrin α3β1, TGF-β signaling pathway is an important target for tumor biological treatment. TGF-β signal pathway of TGF-β receptor Ⅱ (of TβR Ⅱ) first with the ligand of TGF-β combination, followed by of TGF-β receptor Ⅰ (of TβR Ⅰ) to identify and combine to form expression of TβR Ⅱ - ligand composite material, so TβR Ⅰ of GS District serine / threonine residues phosphorylated and phosphorylated TβR Ⅰ then the signal is amplified and passed to the signaling pathway downstream substrate, TβR Ⅰ downstream phosphorylation Co-Smads combine to form heteromeric transferred into the nucleus and transcription factor interactions regulate target gene expression, and ultimately regulate cell migration, invasion, metastasis ability. Latest study, to achieve the purpose of blocking the passage through a variety of methods acts on the target of the receptor protein in the TGF-β signaling pathway, Smads, wherein the TGF-β protein antibody, for the TGF-β gene interference RNA, The research of the role of TGF-β receptor transmembrane protein and small molecule blockers more TGF-β signaling pathway targets are extremely important in the study of tumor biological treatment. Objective: To study the expression of TGF-β1 and role in TGF-β receptor Ⅰ interference RNA (TsiRNA) in vitro on human bladder cancer T24 cells invasion and metastasis of further study the expression of TGF-β1 and TsiRNA migration and invasive ability of protein expression of TGF-β receptor I gene expression and bladder cancer T24 cells, and to explore its mechanism. METHODS: Transwell-migration test, scratch test, as well as Transwell-invasion test observed expression of TGF-β1 and the role of TGF-β receptor Ⅰ interference RNA (TsiRNA) in vitro on human bladder cancer T24 cells invasion and metastasis ability. Western blot (Western Blotting) and RT-PCR to detect TGF-β1 and TsiRNA treatment invasion, migration-related factor gene and protein expression levels change, including MMP2, MMP9, α3, β1, and α2. Statistically SPSS13.0 software Measurement data represents the mean ± standard deviation (Mean ± SD), two sets of measurement data with non-independent samples t-test, and the correlation between each set of indicators using the Spearman rank correlation analysis . P <0.05 was considered statistically significant. Results: In this study, by Transwell-migration assay, scratch test to prove the expression of TGF-β1 can significantly improve the T24 bladder cancer cell migration, and can completely reduce the the migration capacity changes induced by TGF-β1 T24 TsiRNA, Transwell- invasion trials show over expression of TGF-β1 can significantly improve bladder tumor T24 cells in the invasion capacity, while TsiRNA can completely inhibit the role of TGF-β1 in Western Blotting, RT-PCR was the result display through the expression of TGF-β1 can significantly increase the T24 cells integrin subunit α3, β1, α2, and MMP9 expression, while TsiRNA can completely block TGF-β1-induced increase in the amount of the protein molecule expression, such expression changes in the amount of trend and T24 invasion and migration capacity trend was correlation. Conclusion: In this study, people bladder cancer T24 cell line as an object to study the migration of TGF-β1 and TsiRNA bladder tumors, invasive impact, the study found that over-expression of TGF-β1 significantly enhanced T24 cell invasion, migration, the role reduce the invasion of T24 cells by blocking TGF-β signaling pathway in the TGF-β receptor Ⅰ interference RNA migration capacity while reducing integrin protein and MMP9 protein expression, thus proving that the bladder cancer overexpression of TGF-β1 and TsiRNA may completely or partially by regulating integrin protein and MMP9 protein expression affect T24 cell migration and invasion abilities, so applications TsiRNA blocking TGF-β signaling pathway may become invasive bladder tumor biological treatment provide certain theoretical basis.
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