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Natural medicine artemisinin anti- rat liver transplantation immune rejection of

Author: DingHuanXin
Tutor: LiLi；LiXiaoYan；RanJiangHua
School: Kunming Medical College
Course: Surgery
Keywords: Rat Liver Transplantation Model Immune rejection Artemisinin
CLC: R285.5
Type: Master's thesis
Year: 2010
Downloads: 81
Quote: 1
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Abstract


Objective: 1. Establish a stable model of orthotopic liver transplantation through the model, a better skilled microsurgical techniques. (2) on the basis of this model to further explore natural medicine artemisinin-resistant rat liver transplantation immune rejection role in efforts to seek artemisinin resistance acting on the optimal dose of immune rejection by the immune rejection against deeper understanding thus for the future for the clinical treatment of artemisinin resistance after liver transplantation immune rejection to provide a better basis. Methods: two cuff technique for liver transplantation SD-Wistar rat model of experimental animals: Healthy male SD rats as donors, weighing 220 ~ 250 g, male Wistar rats were used as acceptor, weighing 250 ~ 280g, donor weighing slightly less than the receptor 20 ~ 30 g, rat liver transplantation model, and then depending on the time after surgery, respectively, five rats after 3 days, 6 days, 9 days were sacrificed, blood test aspartate aminotransferase (AST) , alanine aminotransferase (ALT), total bilirubin (TBIL), interleukin -2 (IL-2), interleukin -4 (IL-4) and tumor necrosis factor (TNF-α), while liver tissue with HE evaluation of immune rejection staining degree. Results: 1. Liver transplantation in 100 trials, 80 cases survived for more than 3 days, surgical success rate was 80%, in line with the experimental requirements, postoperative survival is not more than three days of rat primary cause of death was bleeding, liver suture Louxue inferior vena cava, portal vein thrombosis and meticulous and vascular thrombosis and a few variations exist and death. (2) After drug treatment, the groups were significantly different compared to the overall (P <0.01), the level of the control group with artemisinin dose group after AST, ALT, TBIL with time were significantly higher in the blank group and Artemisinin and low dose group were significantly different (P <0.01). IL-2, IL-4, TNF-α, compared the two groups there was a significant difference (P <0.01). High and low groups artemisinin IL-4 was increased with time, while the control group showed a downward trend, there was significant difference between the two groups (P <0.01). Cyclosporin A Cyclosporin A group and artemisinin group IL-2, TNF-α, and AST, ALT, TBIL with time decreased, while IL-4 was significantly higher compared to the two groups, the two groups There was no significant difference (P> 0.05). Blank control group and the high and low artemisinin group and cyclosporine A and cyclosporine artemisinin group were significantly different (P <0.01). Cyclosporin A Cyclosporin A group and artemisinin group and high-and low-dose artemisinin There was a significant difference (P <0.01). High and low artemisinin comparison between groups is not obvious. HE staining blank control group and the low-dose group was significantly heavier than artemisinin group of cyclosporine A and cyclosporine A artemisinin group. Blank control groups was more severe in the high and low artemisinin group, the level of artemisinin group than cyclosporine A and cyclosporine A artemisinin group, high, low artemisinin comparison between groups is not obvious. Conclusions: 1. Two cuff technique for rat liver transplantation model infallible, liver transplantation can be used as an effective means of experimental studies, and SD-wistar is an ideal model for immune rejection. 2 artemisinin inhibited IL-2, TNF-α secretion, effectively reduce the degree of rejection. 3 artemisinin and cyclosporine A has obvious synergies with this experiment to reduce the dose of cyclosporine A dose increase artemisinin, can still get satisfactory results. Both full dose with a reasonable amount of cyclosporin A and anti-immune rejection effect is quite specific with ideal dose for further study. 4 artemisinin can effectively promote the expression of IL-4, immune suppression significantly inhibited. 5 medium-dose and high-dose artemisinin still significantly inhibit the occurrence of immune rejection, immune rejection of artemisinin acting on the optimal dose for further study. 6 artemisinin mechanism may resist immune rejection: inhibition of Th1 differentiation may be added to promote the induction of Th2 differentiation appreciation humoral and cellular immunity occur, thereby reducing immune rejection.

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CLC: > Medicine, health > Chinese Medicine > Of Pharmacy > Pharmacology > Chinese medicine Experimental Pharmacology
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