Dissertation > Excellent graduate degree dissertation topics show

Effects of Rapamycin and LY294002 on the Skeletal Muscle of Rat with Swimming Training

Author: HuLiLi
Tutor: LuYaoFei
School: Shanghai Institute of Physical Education
Course: Human Movement Science
Keywords: skeletal muscle rat LY294002 rapamycin exercise
CLC: G861.1
Type: Master's thesis
Year: 2011
Downloads: 23
Quote: 0
Read: Download Dissertation

Abstract


Purpose: Skeletal muscle is an important organ of the body, which is ralated to the exercise abilit and overall metabolic.Exercise can promote skeletal muscle growth and hypertyophy,the study found that PI3K/Akt/mTORsignaling pathway is important to muscle protein synthesis. LY294002 (PI3K inhibitor) and Rapamycin (mTOR inhibitor) play an critical role in the study of the pathway,and widely used in clinical practice.However,the mechanisms that regulate muscle hypertrothy induced by the exercise is poorly understood.The purpers of this study is to establish the exercise-induced muscle hypertyophy model,explore LY294002 and Rapamycin role in the PI3K/Akt/mTOR signaling pathway,and to further enrich the molecular mechnism of skeletal muscle hypertropy in experimental evidence.Methods: 40 male adult Sprague-Dawley rats which load 15% by weight and intermittent swimming training.The subjects are grouped into control group(C),trainning group(T), trainning + LY2940002 group(TL), trainning + Rapamycin group(TR) and trainning + LY2940002 + Rapamycin group(TLR).The inhibitors were injection. The training adopted interval training protocol with load and training every other day with 8 weeks. The biopsy after 8 weeks was taken 24hs after the last trainning.Biopisies were taken from the gastrocnemius and soleus muscles.Using Western Blotting detecte the the protein expession of PI3K/Akt/mTOR and the phosphorylation in the skeletal muscle. Results: After 8 weeks treatment,the body weight of C group was significant higher than others,and no significant was observed in skeletal muscle mass between other groups, but the Soleus and Gastrocnemius Muscle Mass Index in training T group increased significantly compared with control group(p<0.05). Compared with the control group, the ratio of P-PI3K/PI3K、P-Akt/Akt、P-mTOR/mTOR in T group increased with insignificant difference, but the ratio in TR and TRL groups was significantly reduced(p<0.05).Conclusions: These results suggest that the interval training protocol is helpful to increase the relative muscle hypertrophy, and LY294002 and Rapamycin had a inhibition role in the PI3K/Akt/mTOR pathway responding to training.

Related Dissertations

  1. The Third Set of "National Aerobic Gymnastics Training Standards" for Ordinary Routine Primary Health Fitness Female Students in Rats,G831.3
  2. The Study on Institution of New-generation Migrant Workers’ Rights in the Exercise of Security,D412.6
  3. The Influence of Long-term Regular Exercise to the Distribution and Change Regulation on Human Skin Temperature,G804.2
  4. A Study of the Relationship between Physical Exercise and the Optimism of Middle School Students,G804.2
  5. The Effect of Tanshinone ⅡA and Niacin on the Cardiovascular Function of Infant Obese Rat,R285.5
  6. Therapeutical Effects of Diammonium Glycyrrhizinate Lipid Ligand on Nonalcoholic Steatohepatitis and Its Partly Mechanisms of Action,R575.5
  7. Dynamic Effect of Cold Restraint Stress on Damage of Ultrastructure and Expression of Heat Shock Protein in Rat Tissues,S858.91
  8. Effect of in Ovo Leptin Administration on Posthatch Skeletal Muscle Growth in Broiler Chickens,S831
  9. A Practical Research on Cultivating Habits of Junior Middle School Mathematics Exercise Correction and Self Feedback,G633.6
  10. Experiment Study of Ethylhexyl Phthalate (DEHP) and It’s Metabol-ites (Single-ethylhexyl Phthalate, MEHP) Influencingon Sper Matogoniain Young Male Wistar Rats,R329
  11. The Effects of Aerobic Exercise Training on Oxidative Stress and Ultrastructure of Pancreatic Organization in Diabetic Rats,R587.1
  12. Study on Middle-Aged and Elderly People’s Cognitive Beliefs of Exercising,G812.4
  13. Study on the Cell Cycle of Liver Cells during Rat Liver Regeneration,Q253
  14. The Regulatory Role of Proliferation and Apoptosis of ERK1/2 and JNK Signaling Pathways in Eight Liver Cell Types during Rat Liver Regeneration,Q418
  15. BMP2 and VEGF165 Genes Transfected Rat Bone Marrow Mesechymal Stem Cells Simultaneity Expression,R329
  16. The Application Value of Magnetic Resonance Diffusion Tensor Imaging in Skeletal Muscle Tumor,R445.2
  17. Effects of High-salt Dieet on Blood Pressure and the Remodeling of Carotid Arteries and the Intervention of Telmisartan in Wistar Rats,R544.1
  18. The Espression of C-reactive Protein in Rats of the Chronic Obstructive Pulmonary Disease with Diabetic,R563.9
  19. Relationship and Significance of Gene Transcription Profiles in Rat Liver Regeneration and Non-Alcoholic Fatty Liver Disease Occurrence,R575.5
  20. The Beneficial Effects of Soy Isoflavones in Osteoporosis of Ovariectomized Rats,R580
  21. Physical exercise in obese high school students time management tendencies and the impact of academic pressure,G633.96

CLC: > Culture, science,education, sports > Sports > Water,ice and snow sports > Water Sports > Swim
© 2012 www.DissertationTopic.Net  Mobile