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Amperometric Immunosensor and Hydrogen Peroxide Biosensor Based on Nanocomposites

Author: WangJinFen
Tutor: YuanRuo
School: Southwestern University
Course: Analytical Chemistry
Keywords: Immunosensor Human chorionic gonadotropin Biosensor Peroxide Nanocomposites
CLC: TP212.3
Type: Master's thesis
Year: 2010
Downloads: 128
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Abstract


The biosensor is a great potential for development of disciplines, to expand the range of applications in areas such as food, medicine, environment With the biosensor proposed higher requirements, future prospects of sensor applications performance in the following four aspects: Small size, functional diversification, market-oriented, intelligent and integrated. However, the sensor's sensitivity, detection limit and stability factors restricting the development of the sensor. This paper is based on the above consideration, the current biosensors constructed based nanocomposites were prepared to look on the sensitivity and stability, to improve the performance of the sensor. This paper as follows: 1. Prussian blue build layers of self-assembled cysteine ??/ nano-based study of human chorionic gonadotropin immune sensor: the use of electrostatic adsorption layers of self-assembled gold electrode surface cysteine ??/ nano Prussian blue bilayer structure ({NPB / L-cys} 2 ), electrodeposition technology in its surface electric aggregation layer of gold nanoparticles, and then human chorionic gonadotropin fixed on gold nanoparticles its non-specific active sites, and finally closed with hemoglobin, the obtained human chorionic gonadotropin immunosensor. In this paper, the double nano Prussian blue as the immune response in the redox probe, hemoglobin in place of bovine serum albumin as a blocking agent, hydrogen peroxide good electrocatalytic reduction Since the the double nano Prussian blue and hemoglobin, the immune response was significantly be amplified, and compared the experimental results show that the the immune sensor's sensitivity was significantly improved. The transmission electron microscope nanoparticles were characterized using cyclic voltammetry electrode modification process and performance characterization. The results show that the immunosensor measured human chorionic gonadotropin linear range of 0.5-10.0 mIU / mL and 10.0-200.0 mIU / mL, the detection limit of 0.2 mIU / mL. In addition, the sensor also has the advantages of simple preparation, high sensitivity, good stability, long life. Based conductive polymer - carbon nanotube composites to build the current type of human chorionic gonadotropin immunosensor: This article uses gold nanoparticles (GNPs) and poly 2,6 - diaminopyridine / carbon nanotube composite nanomaterials ( pPA / MWNTs) to build a new current of human chorionic gonadotropin the immune sensors (HCG). pPA / MWNTs composites uses the MWNTs surface electrical polymerization of 2,6 - diaminopyridine made, GNPs for HCG antibody fixed in the surface of the composite material. The process of electrode preparation and the performance of the electrode was studied by cyclic voltammetry, electronic scanning tunneling microscope. Since the MWNTs modified surface of the electrode having a large specific surface area can be increased the pPA the fixed amount, redox property of PPA's other hand due to the special nanotube carbon structures and good conductivity can be enhanced. PPA and MWNTs complex, has a synergistic effect can be greatly enhanced the electron transport of the electrode surface, and immune response can be used as a redox probe. In addition, gold nanoparticles having a good conductivity and biological compatibility, the electrode prepared in a linear relationship in the range of 1.0-10.0 mIU / mL and 10.0-160.0 mIU / mL, the detection limit of 0.3 mIU / mL, in addition to the sensor further exhibit good stability and reproducibility. 3 - copper nanocomposites hydrogen peroxide biosensor research: to build a current-based flower-like polymer by polymerization of 2,6 - work aminopyridine and copper sulfate mixed solution prepared flower-like polymer in the surface potential of the gold electrode - copper composite nanomaterials (pPA-FCu), then surface modified by chitosan - zirconia - gold nanoparticles composed of organic-inorganic composite material (ZrO 2 - CS-AuNPs), the last of the Horseradish peroxidase enzyme (HRP) is fixed to the organic-inorganic composite material was prepared HRP / ZrO 2 -CS-AuNPs/pPA-FCu/gold ELECTRODE biosensor. By UV absorption spectrophotometry, scanning electron microscope, cyclic voltammetry, chronoamperometry, and AC impedance method examines the assembly process, electrochemical characteristics and performance of the electrode. The experimental results show that, the Ppa-of FCu composite nanomaterials not only having a good electrochemical redox activity, and hydrogen peroxide have good electrochemical catalysis. The ZrO 2 -CS-of AuNPs composite has good film-forming properties, stability and good biocompatibility. Therefore, the sensor is a good linear relationship in the range of 7.8 × 10-7-3.7x10-3 mo1 / L, the detection limit is 3.2 × 10 -7 mol / L (S / N = 3). Also, its Michaelis constant was 0.32 mmol / L, showing a good affinity. Potential applications in real samples, the biosensor showed good analytical performance, stability and selectivity.

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CLC: > Industrial Technology > Automation technology,computer technology > Automation technology and equipment > Automation components,parts > Transmitter ( converter),the sensor > Biological sensors,medical sensors
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