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The housefly case Insecta , Diptera , houseflies Division . Larvae commonly known as \The housefly larvae crude protein content is high , the proportion of essential amino acids and mode are suitable for human needs , is a very good protein resource ; addition , the body also contains a variety of specific bioactive substances , there is a very good development and utilization value . Housefly larvae powder experimental materials , take the single factor experiment and response surface analysis , antioxidant activity evaluation in vivo and in vitro methods to study the different factors of the antioxidant activity of hydrolysates from enzymatic process , enzymatic in vitro and in vivo antioxidant activity of hydrolysates from major antioxidant component . The results are as follows : 1 . Housefly larvae the best enzymatic process conditions : by Alcalase alkaline protease for the housefly larvae optimal hydrolysis with the enzyme ; enzyme dosage of 3.6 % of the substrate , the optimal pH of 8.2 , enzymatic temperature of 62 ° C substrate concentration of 11.7 % , enzymatic hydrolysis time of 3 h . In the enzymatic hydrolysis conditions the DPPH · housefly larvae hydrolysates from Clear ratio reached 86.39% (10mg/mL). Housefly larvae hydrolysates from DPPH ·, O2-·, · OH scavenging effect as well as reducing ability and concentration was dose-effect relationship , the IC50 for 3.78mg/mL 8.83mg/mL , 1.08mg/mL ; family fly larvae hydrolysates from significantly inhibit the oxidation of linoleic acid peroxide and lard system , add the amount of the increase , the antioxidant effect is more and more obvious . Housefly larvae hydrolysates from SOD activity in serum , liver , and brain tissue can GSH- PX activity, CAT activity significantly improved ( p lt; 0.05 ) significantly reduced the MDA content in these organizations (p lt; 0.05), instructions the housefly larvae hydrolysates from certain antioxidant role . The housefly larvae Hydrolysates through a SephadexG-25 , the optimal separation conditions : sample concentration of 20mg/mL, eluent of deionized water , the elution rate 0.5mL/min , loadability 1mL . Under this condition , the housefly larvae hydrolysates by SephadexG-25 separated the two components can be obtained , was measured to the highest antioxidant activity of the component 2 , the molecular weight range for 2.3KDa 1.2KDa , DPPH · Clear rate reached 88.15 %. Description the housefly larvae of hydrolysates from antioxidant activity is closely related to its molecular size .
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