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Preparation and Application of a Water-Soluble Visible-Light-Sensitized Luminescent Europium Complex

Author: JiangLiNa
Tutor: YuanJingLi
School: Dalian University of Technology
Course: Analytical Chemistry
Keywords: Europium Complex Water-soluble Visible light excitation Environmental pathogenic microorganisms Time-resolved fluorescence bioimaging
CLC: O657.3
Type: Master's thesis
Year: 2010
Downloads: 98
Quote: 1
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Abstract


The fluorescence characteristics of rare earth fluorescent biomarkers probe long life and developed a highly sensitive time - resolved fluorescence biochemical analysis technology has been widely used in clinical testing and the field of biotechnology . However, due to the need to use most of the rare earth phosphor biomarkers phototoxicity larger UV as the excitation source , the biological sample of the living body has a certain light damage , and several long-wavelength excitation of europium complexes currently known and there is a water - soluble , polar coordinating solvent is unstable , the luminescence quantum yield is low , or the lack of active marker groups such problems can not be used for biological markers , thus limiting the time-resolved fluorescence biochemical analysis techniques in the living body sample detection analysis . This study by two ligand 4,4 '- (1 \chlorosulfonyl \1 - yl ) -1,3,5 - triazine (DPBT) together with europium ion ligand , having a visible light excitation performance of the ternary europium complexes with BHHCT - of Eu3 - DPBT prepared , and then through the complexes with bovine Serum albumin , prepared a water-soluble and can be visible light excitation europium complexes binding protein . A fluorescence excitation wavelength may be extended to the visible zone ( gt ; 380 nm ) of the binding protein , and having a high fluorescence quantum yield ( 27%) , and the longer the fluorescence lifetime (0.46 ms), the binding protein labeled streptavidin prime after successfully used for immunofluorescent staining of pathogenic microorganisms in water samples Giardia lamblia lamblia (Giardia lamblia), mouse Cryptosporidium (Cryptosporidium muris) , as well as the small saphenous Cryptosporidium (Cryptosporidium parvium) and time-resolved fluorescence determination of micro- imaging . The results show that this study, preparation of water-soluble visible light excitation the europium complexes binding protein can be used as a new type of fluorescent markers for time-resolved fluorescence biological imaging analysis field .

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CLC: > Mathematical sciences and chemical > Chemistry > Analytical Chemistry > Instrument analysis ( physics and physical chemistry ) > Photochemical analysis ( spectral analysis method)
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