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Endogenous metabolites X raised a cyclinD1 promote C_2C_ (12) through the activation of the ERK pathway cell proliferation

Author: LiLi
Tutor: ZhuDaHai；ZhangYong
School: Peking Union Medical College , China
Course: Biochemistry and Molecular Biology
Keywords: Endogenous metabolites Cell proliferation ERK cyclinD1
CLC: Q26
Type: Master's thesis
Year: 2010
Downloads: 39
Quote: 0
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Abstract


In 1923 , Warburg found that the rapid proliferation of tumor cells is mainly metabolized through glycolysis , known as the Warburg theory . Warburg theory in recent years more and more people to seek and find new biological function of endogenous metabolites or related enzyme has become a hot spot of today 's life science research . Recent growth rate , the laboratory there were significant differences of laying hens and broiler Proteome and Transcriptome comparison study found that the rapid growth of broiler muscle tissue regulate X metabolic enzyme Z, expression , and enzyme activity was significantly higher than the hens muscle is the major organs of endogenous metabolites X , the results suggest that endogenous metabolite X with the the ontogenetic the skeletal muscle growth rate some contact . Therefore , this paper studies the impact of of endogenous metabolites X on skeletal muscle cell proliferation and the possible molecular mechanisms . Cell Biology Experimental results show that endogenous metabolite X promote C2C12 muscle cell proliferation , further research found that X may be through the activation of the ERK pathway in the transcriptional and translational levels raised cyclinD1 expression , and thus play a role in promoting cell proliferation . More importantly, the endogenous metabolites X may be synergistic IGF1 play a further role in promoting the proliferation of cells , the same time , X can also antagonize MSTN the inhibitory effect on cell proliferation . In this study, do not express the HepG2 cells and X- Z - enzyme product downstream of the A preliminary study of the X promote cell proliferation is dependent on their metabolic functions . The results show that the endogenous metabolites X can promote not express Z enzyme proliferation of HepG2 cells , and its downstream metabolites A on the proliferation of C2C12 cells useless showed the promoting effect . These results suggest that X may not rely on its own metabolic pathway promote skeletal muscle cell proliferation . In summary , our experimental results show that , the the endogenous metabolite X may occur in skeletal muscle and has a very important regulatory functions in the regeneration process , and is expected to develop into a potential drug for the treatment of skeletal muscle and metabolic diseases .

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