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Expression and Significance of HPV、 p15、 EGFR and Bax in the Sinonasal nverted Papiloma and Nasal Squamous Cell Carcinoma

Author: LiuMingNa
Tutor: WuXianGuang
School: Shantou University
Course: Otorhinolaryngology
Keywords: Sinonasal inverted papilloma Squamous cell carcinoma human papilloma virus p15 CyclinD1 EGFR Bax immunohistochemistry
CLC: R739.62
Type: Master's thesis
Year: 2011
Downloads: 13
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Abstract

ObjectiveTo investigate the expression of HPV、CyclinD1、p15、EGFR and Bax proteins in thesinonasal inverted papilloma、nasal squamous cell carcinoma and inflammatory nasal polyptissues,study the roles and relationships in the occurrence process of the sinonasal invertedpapilloma and nasal squamous cell carcinoma.Materials and MethodsParaffin blocks of surgical specimen of the tumors of nasal cavity and sinus and intranasalbiopsy, including 11 cases of inflammatory nasal polyp(INP),34 of the sinonasal invertedpapilloma (SNIP),15 of nasal squamous cell carcinoma(SCC) were selected from Shantouuniversity medical college pathology staff room and Dept.of pathology of second affiliatedhospital from 1999 to 2009. Expression of HPV、CyclinD1、p15、EGFR and Bax proteins weredetected by immunohistochemistry Envision two-steps method in 60 tissue samples. Statisticalanalysis was performed using SPSS17.0. Chi-square test and Fisher’s exact test were used toobtain statistically significant differences between two groups. Pearson correlation coefficientswere calculated to evaluate the relationships among proteins. The correctedα=0.05. A value ofP<0.05 was considered statistically significant. A value of P<0.01 was considered highlystatiscally significant.Results1. Pathological observations：3 cases of 34 cases SNIP were pseudostratified ciliated columnar epithelium papilloma, therest were squamous papilloma; the epithelials of 3 cases had moderate and severe cervicalsquamous cell dysplasia. 1 case of 15 cases SCC was with a little inverted papillomaorganization, the rest 14 cases were simple squamous cell carcinoma.2.Immunohistochemical tests： ⑴Under the light microscope, the positive area of HPV protein was located in the nuclears,showing buff or shallow brown; the positive areas of CyclinD1 and p15 proteins were locatedin the nuclears, showing brown-yellow or brown; the positive areas of EGFR protein waslocated in the membranes, showing brown-yellow; positive areas of Bax protein was locatedin the cytoplasm showing light yellow or dark brown.⑵①Positive expression rates of HPV protein were respectively 0%、38.24%、26.67% in theINP、SNIP、SCC. The difference between SNIP and INP was statistically significant(χ2=5.915，P=0.018<0.05).The rest were no statistically significant among groups(P>0.05).②Positive expression rates of CyclinD1 protein were respectively 18.18%、35.29%、66.67% inthe INP、SNIP、SCC. The difference between SCC and INP was statistically significant(χ2=6.003，P=0.017<0.05).The rest were no statistically significant among groups(P>0.05).③Positive expression rates of p15 protein were respectively 90.91%、35.29%、26.67% in theINP、SNIP、SCC. The differences between SNIP and INP and between SCC and INP werehighly statistically significant(χ~2=10.288，P=0.001<0.01；χ~2=10.539，P=0.001<0.01).Thedifference between SCC and SNIP was no statistically significant (P>0.05).④Positive expression rates of EGFR protein were respectively 18.18%、79.41%、93.33% in theINP、SNIP、SCC. Significant differences were obtained between SCC and INP (χ~2=15.143，P=0.00016<0.01) and between SNIP and INP (χ2=13.599，P=0.00048<0.01).Thedifference between SCC and SNIP was no statistically significant (P>0.05).⑤Positive expression rates of Bax protein were respectively 81.82%、70.59%、33.33% in theINP、SNIP、SCC. The differences between SCC and INP and between SCC and SNIP werestatistically significan（tχ~2=6.003，P=0.017<0.05；χ~2=5.980，P=0.026<0.05）。The differencebetween SNIP and INP was no statistically significant (P>0.05).⑥No significant difference was found among the expression of HPV、CyclinD1、p15、Bax、EGFR five kinds of proteins in the SNIP and SCC（P>0.05）.Conclusions1. There is relationship between HPV infection and the occurrence of SNIP, to further prove that HPV infection is one of important etiological factors in the occurrence of SNIP and HPVis an important index in the occurrence of SNIP.2. There is relationship between over-expressed of CyclinD1 protein and SCC; it suggests thatover-expressed of CyclinD1 protein may play an important role in the process of theoccurrence of SCC. High expression of CyclinD1 protein may has the trend to promote thecancerous of SNIP, but it still needs to expand in sample size to further confirmation;3. There are relationships between low expression of p15 protein and SNIP and SCC; itsuggests that low expression of p15 protein may play important roles in the process of theoccurrence of SNIP and SCC;4. There are relationships between excessive expression of EGFR protein and SNIP and SCC; itsuggests that excessive expression of EGFR protein may play important roles in the processof the occurrence of SNIP and SCC;5. There is relationship between significantly low expression of Bax protein and SCC; itsuggests that low expression of Bax protein may have a very importantly negative regulatingrole in the canceration of SCC and indicate that SNIP has the trend of canceration;6. The expressions of HPV, CyclinD1, p15, Bax and EGFR protein have no correlations in theSNIP and SCC, and this needs to be further study;

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