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Comparative Study of Biocompatibility between PLAG/HA and Allogenic Bone in Vitro

Author: LiZhongYi
Tutor: TuGuanJun
School: China Medical University
Course: Surgery
Keywords: Rabbit marrow stem cell PLGA/HA scaffolds allogenic bone tissue engineering
CLC: R318.08
Type: Master's thesis
Year: 2010
Downloads: 40
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Abstract

objectiveStudy the biocompatibility of PLGA/HA in vitro, contrast with the allogenic, bone.detecting the adhesive and multiplying activity on the surface of PLGA/HA validate the osteoblastic of the cell on the surface of biocompatibility.compare the differences between PLGA/HA and allogenic bone.Resultrabbit BMSCs can development both on the surface of PLGA/HA and allogenic bone, the cells can form calcium nodules after induced in vitro.it full prove that the adhesive capacity and activity on the surface of PLGA/HA is not lower than those on the surface of allogenic bone.There is no significant difference between the two groupsMethodMSCs are taken from marrow of 3 Newsland rabbits. then isolated in vitro, cultured proliferated and purified. Take the third generation of BMSCs, making the cell concentration 1*106/ml, Seeding them on the surface of PLGA/HA and allogenic bone until saturated.Put the culture board in the culture box with 37。C and 5%CO2 for 4 hours, then seed the cells in the same way, make sure there are 1*105cells, after that add inducing culture fluid and put the materiols in the box, change the fluid each other day.observe the formation and adhesion of the two generations every day.4 hours after seeding, take 2 sample boards from each materiols, count the number of adhesive cells, we can get the adhesion rate from the formula [adhesion rate=number of adhesive cell/number of seeding cell]* 100%. Similarly, count the cells of 2 boards respectively from the two groups 3 and 7 days after seeding, take the average and compare the multiplying activity.7 and 14 days after seeding separately, select 2 holes of esch group, detecting the activity of alka line phosphatase.Add tetracycline hydrochloride of lOμg/ml to the culture fluid, exclude the bacteria,14 days later exchange it with the former fluid,24 hours later observe the calcium nodules using fluorescence microscope.Analysis of date to detect the adhesion rate and activity of alka line phosphatase and make t examinationResult①5 days later observing the cell through microscope we can see the cell growth adherent to the culture flask and form further clones.RBC was eliminated by changing the culture fluid. The cell is fibro-cell-liked, triangle shaped, and arranged in spiral or radial.②detect of adhesion rate:4 hours after cell seeding, adhesion rate:PLGA/HA is 33%. allogenic bone is 30%③3 and 7 days later, the multiplying rate of two groups have no significant difference④in the 7th day detecting the activity of alka line phosphatase(ALP), there is no significant difference between two groups⑤we can see the calcium nodules in both group and grow bigger day by dayConclusionthe activity of protein and ALP exist in both group and can multiply, excrete and express after cultured the BMSCs combined with PLGA/HA and allogenic bone. It full prove that the adhesive capacity and value-added activity on the surface of PLGA/HA are not lower than those on the surface of allogenic bone.It lay a good foundation for the in vivo test of animals about PLGA/HA.

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CLC: > Medicine, health > Basic Medical > Medical science in general > Biomedical Engineering > General issues > Biomaterial
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