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The the MxA gene encoding the MxA protein having a broad-spectrum anti - viral activity , an amino acid change will cause a change of its antiviral activity . Mongolian horse, strong disease resistance , so its MxA gene polymorphism analysis and build the Mongolian horse MxA gene eukaryotic expression vector for equine animal resistance gene selection , MxA protein antibodies developed , the production of antiviral protein MxA drug to develop resistant animals by transgenic technology to lay a theoretical foundation . In this study, the local varieties Mongolian horse for the study, published in GenBank horse MxA gene primers were designed horse ujimqin , the uxin horse barag horse , tin Nihe , horse Mongolian horse different groups of blood extraction DNA, was amplified gene fragment of exon 7 , and then PCR-SSCP polymorphism analysis , found that only ujimqin horse MxA gene exon III polymorphism , MxA gene cDNA sequence first 429 point mutation from cytosine (C) to guanine ( G ) , causing the corresponding amino acid changes , i.e. , leucine (Leu) into tyrosine ( Tyr ) , the rest were not found polymorphism . RNA was extracted from peripheral blood mononuclear cells of Mongolian horse , using RT - PCR ( reverse transcription polymerase chain reaction ) method to get MXA cDNA . Amplified gene target fragment by agarose gel electrophoresis recycling , and pGEM ? - T Easy vector connection , and transformed into E. coli , and screening recombinant the digested purpose of gene MXA cDNA was cloned into the eukaryotic expression vector pCI - neo , was identified by PCR , confirmed inserted into the vector PCI - Neo containing the nucleotide sequence of the target gene eukaryotic expression recombinant plasmid pCI - neo MXA cDNA was successfully constructed .
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