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Rhododendron is a highly ornamental rhododendrons , mainly by seed propagation . Natural low germination rate of the seeds , the slow rate of reproduction , in addition to serious damage to their growth environment , Rhododendron has been to the extent of up to endangered species . In this study, the use of tissue culture methods be cultured in vitro , can make the continuation and development of endangered Rhododendron , has a very important significance . In addition , this study also analyzed from the anatomical and molecular level altitude morphological , structural and genetic diversity , and to provide a theoretical basis for the protection and development of Rhododendron . Derived through Rhododendron tissue culture research : best induction medium WPM 8 mg / L ZT 3 mg / L IBA , pH = 5.2 , additional sugar 20g / L ; Best Rooting medium ( ? ) VPM 0.4 mg / L IBA 0.1 mg / L NAA 1g/LAC; the best transplanting matrix moss soil , transplanting survival rate of 94% . On the the Rhododendron leaf anatomical results of the research results show that : using an optical microscope of Changbai Mountain altitude of 2570 m , 2381m , 1982 m , at different heights of 1788 m and 1695 m in Dunhua old Hakusan growth of Rhododendron leaves crosscut surface observation . The observed results reduced gradually as the altitude increases blade external morphology , leaf internal structure of the blade diameter of the main vein , palisade tissue thickness , the thickness of the upper epidermis gradually increase . Preliminary study showed that the diversity of Rhododendron RAPD technique : the the the RAPD reaction optimization system for Rhododendron : total volume of 20 μL PCR reaction containing template DNA10 ng Mg2 1.5mmol / L , primers , 15 pmol / μL , dNTP 0.15 mmol / L, Taq DNA polymerase, 1.0U. Five different altitude groups , the genetic diversity analysis showed that the genetic similarity coefficient of 0.80 , five Rhododendron groups can be divided into two categories , the first category Jilin Changbai Mountain , the second category is the Jilin old Hakusan , a preliminary understanding of the genetic relationship between the different groups of rhododendron distance .
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