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Screening of High Production Strains of Cordyceps Militaris and Optimizing Its’ Fermentation Process

Author: DiJingBo
Tutor: ZuoLiRong
School: Jilin University
Course: Pharmaceutical Analysis
Keywords: Cordyceps Militaris Mutation screening Optimization Fermentation process
CLC: S567.35
Type: Master's thesis
Year: 2010
Downloads: 437
Quote: 3
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Abstract


Cordyceps has anti-tumor, anti-fungal, anti-inflammatory, anti-fatigue, anti-aging and regulation of the immune pharmacological activity and biological activity, good treatment and the effectiveness of health care by the great concern of the people, but because of the wild Cordyceps resources limited, and the domestication and artificial culture research has been slow. So people's eyes turning Cordyceps have similar efficacy and content of some bioactive substances the high cross Cordyceps Cordyceps Militaris Cordyceps Militaris domestication and artificial solid culture and submerged fermentation. Long fermentation period, however, the low yield of mycelium or low content of mycelium of active ingredients, and still can not meet the demand of the people for cordyceps growing, Cordyceps Militaris fermented improvement research has important implications, this article from bacteria improvement and fermentation process optimization of the two-pronged approach to improve the fermentation of Cordyceps Militaris, and achieve better results. Using nitrosoguanidine and of Laser Mutation of Cordyceps Militaris CGMCC 5.699: mutagenesis, screening mycelium growth speed and colony size, and then considering the mycelial dry weight mycelium adenosine, respectively Cordyceps militaris polysaccharide and Cordyceps acid content, the shake-flask method rescreening, the screened yield Cordyceps Militaris mutant continuous passage for genetic stability study, to obtain a high yield Cordyceps Militaris mutant strains. Single factor test Preliminary to optimize yield Cordyceps Militaris mutant fermentation medium, the Plackett-Burman experimental design, adenosine content as an indicator, the multivariate linear regression model analysis, significantly affect the fermentation medium screened group points, and then to examine factors significant component of the center of rotation of a combination of design experiments, response surface analysis adenosine content in response to value, to obtain high yield of Cordyceps Militaris best fermentation medium. Single factor test method considering mycelial dry weight adenosine content in Cordyceps militaris polysaccharide content and Cordyceps acid content, optimize yield Cordyceps Militaris strains 5 L tank fermentation conditions, to obtain the best 5 L fermentor optimum fermentation conditions, validation experiments in this under optimal conditions, the results showed that the yield of Cordyceps militaris mutant culture conditions optimized, stable growth, little change in the dry weight of the mycelium and active ingredient content. Investigated yield Cordyceps Militaris mutants 15 L fermentor and 100 L fermenter Cultures, the results showed that the yield of Cordyceps Militaris mutants in the enlarged training process, the active ingredient in the mycelium and mycelium in varying degrees of improved, and the fermentation time is shortened.

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CLC: > Agricultural Sciences > Crop > Economic crops > Medicinal crops > Fungus > Cordyceps sinensis
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