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Influence of VEGF on MMP-2 Expression of H460 Cells in Three-dimensional Culture Collagen Gels

Author: GuoXiaoBin
Tutor: ZhuYunKui
School: Lanzhou University
Course: Department of Respiratory Medicine
Keywords: Vascular endothelial growth factor Matrix metalloproteinase-2 Invasion and metastasis of lung cancer Cells in three-dimensional culture model Gelatin zymography
CLC: R734.2
Type: Master's thesis
Year: 2010
Downloads: 40
Quote: 0
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Abstract


Lung cancer is the highest morbidity and mortality in the world today malignant tumor invasion and metastasis is the leading cause of death of patients with lung cancer . Invasion and metastasis of lung cancer is closely related to the matrix metalloproteinase and vascular endothelial growth factor and other factors . The matrix metalloproteinases are a group of zinc-dependent endopeptidase , tumor and stroma sources , matrix metalloproteinases are available through most of the degradation of the extracellular matrix and basement membrane composition affect the occurrence , development and metastasis of the tumor . Matrix metalloproteinase- 2 can degrade the basement membrane type IV collagen and involved in angiogenesis , and tumor closely related . Vascular endothelial growth factor is the strongest specificity involved in the angiogenesis positive regulatory factors , the study found a variety of tumor cells express VEGF protein , such as prostate cancer , lung cancer , stomach cancer, breast cancer . This article studies the lung MMP-2 and VEGF expression and function reveal the mechanism of invasion and metastasis of lung cancer , can provide important reference for the evaluation of lung cancer invasion, metastasis and prognosis . Objective To establish H460 cells three dimensional culture model to investigate the impact of VEGF on the expression of MMP-2 in H460 cells . Non-small cell lung cancer ( NSCLC ) H460 cell lines of lung cancer cells in three-dimensional culture model , were randomly divided into three groups , the determination of the three groups to give different concentrations of VEGF , gelatin zymography of MMP-2 expression results ( 1 ) group H460 cell growth ; ( 2 ) in each experimental group of H460 cells , the intracellular expression of MMP-2 in different concentrations of VEGF and the role of the different time difference . Dimensional culture after 24h hours of each group ran supernatant gelatin zymography , MMP - 2 plasminogen activating enzyme activity have varying degrees of enhanced with the increase of concentration of VEGF 48h -hour , three-dimensional culture supernatant was run gelatinase spectrum , the high concentration of VEGF , MMP - 2 plasminogen activating enzyme activity increased, and expression has been enhanced over 24h . Conclusion ( 1 ) of VEGF up-regulate the expression of MMP-2 in H460 cells , and promote the role of a dose - time-dependent ; higher VEGF concentration , the longer , the stronger the expression of MMP - 2 and its activating enzyme . Promote tumor growth and metastasis , and the promotion of MMP-2 expression . (2) three-dimensional culture model is conducive to the observation and study of matrix metalloproteinases in tumor invasion and metastasis .

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CLC: > Medicine, health > Oncology > Respiratory system tumors > Lung tumors
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