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Expression and Clinical Significance of Bcl-xl in Human Astrocytoma

Author: SunHuaiYu
Tutor: WangYunJie
School: China Medical University
Course: Surgery
Keywords: Astrocytoma Inhibitor of apoptosis proteins Bcl-xl Gene Expression
CLC: R739.4
Type: Master's thesis
Year: 2010
Downloads: 18
Quote: 0
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Abstract


Objective To study the apoptosis inhibitory protein Bcl-2 family members Bcl-xl in human astrocytoma expression and different grade astrocytomas relationship between clinicopathological features for early diagnosis and treatment at the genetic level provide a theoretical basis. Methods of collecting 30 cases of different levels of human astrocytoma tissues and 8 cases of normal brain tissue samples (including 5 grade Ⅰ, Ⅱ grade 15 cases, Ⅲ - Ⅳ grade 10 cases), (8 cases of normal brain tissue as the internal pressure surgery to get), specimens were obtained from January 2008 - January First Affiliated Hospital of China Medical University, surgical resection and after pathological diagnosis confirmed tumor grade meet 2001 WHO Guidelines on human glioma grading standards. Reverse transcription polymerase chain reaction (RT-PCR) detection of Bcl-xl mRNA expression levels were detected by Western blot and Bcl-xl protein expression. Applied Statistics SPSS software for statistical analysis between the two groups were compared using t test, two samples compared with the rate x2 test methods to determine the normal brain tissue and human astrocytoma tissue expression of Bcl-xl whether there are differences, but also to determine different levels of astrocytoma tissue expression of Bcl-xl whether there are differences. Results gray gel image analysis system analysis, bcl-xl gene expression values ??(bcl-xl optical density / β-actin absorbance value) × 100% representation. 30 cases of human astrocytoma 29 cases with Bcl-xl mRNA and protein expression, 8 cases of normal brain tissue six cases have Bcl-xl mRNA and protein expression, astrocytoma tissues and normal brain tissue bcl- xl mRNA expression rates were 96.7% and 75%, respectively, Bcl-xl gene expression was no statistical difference between the two proteins no statistically significant difference between the rate. However, Bcl-xl in astrocytoma gene and protein expression levels were significantly higher than in normal brain tissue levels (P lt; 0.01), there are significant differences between the two groups. Between different levels of astrocytic expression of Bcl-xl were significantly different, according to 2001 WHO Guidelines on human astrocytoma grading standards, higher tumor grade, Bcl-xl gene and protein expression level is higher. Conclusion The human brain astrocytoma Bcl-xl expression at the mRNA and protein levels compared to normal brain tissue were significantly increased, Bcl-xl at different grade astrocytomas expression differences, indicating that Bcl-xl may be associated with tumor cell active on the generation and proliferation. Numerous studies have demonstrated, Bcl-xl in a variety of malignant tumors have specific expression and inhibition of apoptosis through a variety of ways. Therefore, reducing the human brain astrocytoma of Bcl-xl levels, improve immune cell tumor Bcl-xl level, it will be the treatment of human astrocytoma of a new way.

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