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Effects of Oligosaccharides on the Proliferation of a H2O2-producing Lactobacillus from Human Vagina in Vitro

Author: LiangAiLing
Tutor: YangZuoQin
School: Lanzhou University
Course: Obstetrics and Gynaecology
Keywords: Oligosaccharides Lactobacillus H2O2 Proliferation
CLC: R711.3
Type: Master's thesis
Year: 2010
Downloads: 41
Quote: 0
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Abstract


Purpose H2O2 in vitro proliferation of lactobacilli vaginal birth through the observation of oligosaccharides , oligosaccharides and Lactobacillus made ??more effective prevention and treatment of reproductive tract infections Compound lemonal laboratory evidence . Isolated from healthy women vaginal a production H202 the advantages of lactic acid bacteria , their biochemical identification ; through determination of the change of the cultured liquid OD values ??to observe three different types and concentrations of oligosaccharides The strains Lactobacillus vitro proliferation impact. Results a production of H2O2 advantage lactobacilli isolated from healthy women with vaginal identified to curl Lactobacillus , the optimal pH for growth pH7, to inhibit the growth of E. coli DH5a , and Staphylococcus aureus in vitro antagonistic test . The lactic acid bacteria of the strains produced H2O2 in the OD value of the culture liquid with the three oligosaccharide concentration increases gradually increased, the net increase in the the culture liquid Isomaltooligosaccharide containing three different concentrations of the OD value . Conclusion vaginal birth H2O2 curl lactobacilli can take advantage of Isomaltooligosaccharide stachyose , the fructooligosaccharides three oligosaccharides as carbon source for growth ; Isomaltooligosaccharide stachyose fructooligosaccharides three oligomeric the sugar effective promote vaginal birth a H2O2 curl Lactobacillus vitro proliferation .

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CLC: > Medicine, health > Obstetrics and Gynaecology > Gynecology > Female genital inflammation
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