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Korean pine ectomycorrhizal fungi and litter decomposition Correlation

Author: LiuYuanKai
Tutor: DuChunMei；SongFuQiang
School: Heilongjiang University
Course: Microbiology
Keywords: Ectomycorrhizal fungi Korean pine Litter Nutrient cycling Correlation Analysis
CLC: S718.81
Type: Master's thesis
Year: 2010
Downloads: 98
Quote: 0
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Abstract


The ectomycorrhizal (Ectomycorrhiza) mutualism soil mycorrhizal fungi and higher plants root formation. Korean pine is the ectomycorrhizal dependent species, at the same time there is a lot of litter in the Korean pine forest vegetation, its decomposition is an important part of the ecosystem material cycling, litter fungal decomposition release nutrients to be absorbed and used by the plant roots . Tree growth and litter decomposition close link between metabolism and mycorrhizal fungi. Thesis investigate acquisition of Korean pine forest ectomycorrhizal fungi, and initially identified the characteristics of the culture of the ectomycorrhizal fungi, pot experiment by designing the infection rate of pine seedlings, plant biomass, plant growth process in the litter remaining N , P, K content, and soil nutrient substrate main nutrient elements change indicators for the analysis of Korean pine ectomycorrhizal fungi tries to reveal the correlation between litter decomposition and Korean pine forest. The experimental results show that the investigation Forest National Nature Reserve small Xing An Lingfeng Korean pine forest in Macrofungi 6 Section 8 are 22 kinds of ectomycorrhizal fungi were collected. Isolated 14 species of wild mycorrhizal cultural characteristics analysis results show that: most strains are the most suitable grown on glucose as the carbon source, peptone as nitrogen source PDMA and MMN medium. 14 mycorrhizal fungi tested mostly in partial thermophilic bacteria, its optimum temperature at about 31 ℃, the few several strains to low-temperature bacteria growth in the range of 25 ℃ ~ 26 ℃ best. The majority of bacteria grow best between pH5-7 is acidic neutral bacteria. Application response surface methodology ultimately determine the the Suillus grevillei strain optimized medium formulation KH2PO4 to 0.3 g / L, peptone 16g / L glucose 12g / L,. Analysis of the changes in flux magnitude characteristic of Korean pine mycorrhizal fungi and litter decomposition process nutrients pass, pine ectomycorrhizal fungi mixed litter training to improve the pine seedlings soil matrix N, P, K nutrients absorption and utilization of composite culture treatment effect was significantly better than the single-inoculated with mycorrhizal processing and control processing; and composite culture processing speed up of litter N, P and K nutrient release process, promote pine seedlings on litter nutrients utilization. Mycorrhizal fungi and litter mixed-culture treated pine seedlings in height P nutrient conditions the stress (0mg/kg) conditions, more significant effect on the the litter nutrient soil matrix nutrient utilization.

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CLC: > Agricultural Sciences > Forestry > Forestry basic science > Forest Biology > Forest Microbiology > Fungi
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