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Injection of Autologous Muscle Stem Cells (Myoblasts)for the Treatment of Denervated Vocal Fold Paralysis

Author: HouXiaoYan
Tutor: SunJingWu
School: Anhui Medical University,
Course: Otorhinolaryngology
Keywords: Myoblast Glottal insufficiency Amyotrophic Transplant
CLC: R767
Type: Master's thesis
Year: 2010
Downloads: 8
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Abstract

Objective: To observe the rabbit after recurrent laryngeal nerve cut the myoblast transplantation ability to slow down the thyroarytenoid muscle atrophy , and the promotion of renewable motor endplate . Methods: Unilateral loss of the recurrent laryngeal nerve animal model 20 rabbits were randomly divided into two groups , were cut off the left recurrent laryngeal nerve . Into take experimental group sternocleidomastoid muscle cells cultured under sterile conditions (about 10 g ) were isolated and cultured myoblasts by enzymatic digestion , cell expansion to January 1 × 106 cells / ml injection in loss of laryngeal recurrent nerve thyroarytenoid muscle and vocal cord . The control group was injected with saline . Cells cultured in vitro process , its identification of desmin and α - actin immunohistochemistry . Animal feeding 2 weeks and 2 months after the animals were killed , measurement bilateral thyroarytenoid muscle wet weight HE staining and acetylcholinesterase histochemical staining were observed . Motic image advanced 3.2 image analysis system for determination of muscle fiber cross-sectional area . The experimental group and the control group using the surgery side autologous contralateral ratio of the measured values ??obtained survival rate loss of nerve thyroarytenoid muscle wet weight lost nerve thyroarytenoid muscle cross-sectional area of ??the residual rate . The data obtained are expressed as mean ± standard deviation , using SPSS 11.5 statistical software analysis, line two sets of samples t-test . Results : Two weeks and eight weeks , measuring bilateral thyroarytenoid muscle wet weight , draw the thyroarytenoid muscle wet weight residual rate , the experimental group were 74.28% ± 8.39% and 45.37 % ± 6.78% of the control group were 53.57 % ± 2.52% and 27.89 % ± 3.14 % thyroarytenoid muscle fiber cross-sectional area of ??the residual rate of the experimental group were 70.55% ± 7.12% and 57.76% ± 6.27%, and the control group were 52.23% ± 4.76% and 32.63% ± 3.41% ; experimental group than the control group , the difference was statistically significant ( P lt; 0.05 ) . Summary: myoblast transplantation can reduce the loss of nerve thyroarytenoid muscle atrophy , provide treatment method for incomplete missing nerve glottis closes

Injection of Autologous Muscle Stem Cells (Myoblasts)for the Treatment of Denervated Vocal Fold Paralysis

Abstract

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